Clinicians should instead rely on clinical impression and laboratory diagnosis by rRT-PCR.Lucas, P. MMorgan, O. WGibbons, T. FGuerrero, A. CMaupin, G. MLucas PM, et al. Diagnosis of 2009 pandemic influenza A (pH1N1) and seasonal influenza using rapid influenza antigen tests, San Antonio...
One-way ordinary ANOVA test was used for statistical analyses. g Gel analysis of IC Nluc virus stability. The left panel depicts the theoretical results of RT-PCR followed by restriction enzyme digestion. The right panel shows the gel analysis of the RT-PCR products before (lanes 1–3) and...
Fast, high-throughput methods for measuring the level and duration of protective immune responses to SARS-CoV-2 are needed to anticipate the risk of breakthrough infections. Here we report the development of two quantitative PCR assays for SARS-CoV-2-specific T cell activation. The assays are ...
analysis, we programed the instrument for automated analyses of the serum antibody levels from donors who have received two doses of different vaccines, as well as those from donors who were unvaccinated but not infected by Covid-19 (confirmed by the PCR tests of their oropharyngeal swab ...
The obtaining of the cDNA by reverse transcrip- tion, the cDNA amplifying by PCR and cloning of the cDNA fragments in pGEM-T easy vector (Promega) were done essentially as described by Darissa et al. [15], using the single-primer amplification technique (SPAT). For the C...
EasyCov test was assessed under double blind clinical conditions (93 asymptomatic healthcare worker volonteers, 10 actively infected patients, 20 former infected patients tested during late control visit). EasyCov results were compared with classical laboratory RT-PCR performed on nasopharyngeal samples....
P-values between significantly different IAV groups for individual RIDTs were determined by Tukey's honest significant difference test. All analyses were performed in Microsoft Excel 2010. Results Comparison of ID50, Nucleoprotein, and RT-PCR Ct measures Figure 1 lists the ID50, Ct values, and ...
We tested nasal wash samples from 1 April to 7 June 2009 from 1538 patients using the QuickVue Influenza A+B (Quidel) rapid influenza antigen test and compared the results with real-time reverse transcription polymerase chain reaction (rRT-PCR) assay (gold standard). The prevalence of 2009 ...
randomly chosen isolates relative to the PA01 reference strain by quantitative reverse transcription PCR (qRT-PCR). This clone of ST17 has previously been reported as the cause of a nosocomial outbreak at the Virgen Macarena hospital35. Fig. 2: Genomic data and isolate phylogeny....
Real-time PCR also boasts high specificity, a wide detection range, and the ability to detect single DNA copy differences, making it an ideal candidate as a rapid diagnostic test. Moreover, it aligns with the WHO’s ASSURED criteria, being affordable, sensitive, specific, user-friendly, rapid...