There are different types of RNATs: single-stranded DNA probe assays (ssDNA) [15], real-time quantitative PCR (qPCR) [16], loop-mediated isothermal amplification (LAMP) [17], helicase-dependent amplification (HDA) [17] and nicking-enzyme amplification reaction (NEAR) [18]. RNATs seem to ...
Abbreviations EDTA: Ethylenediaminetetraacetic acid HRP: Histidine rich protein LAMP: Loop-mediated isothermal amplification NPV: Negative predictive value PCR: Polymerase chain reaction PPV: Positive predictive value PURE: Procedure for ultra-rapid extraction RDT: Rapid diagnostic test ...
The performance of this assay was compared to the established POC Binax NowFlu A and NowFlu B test, and the reference diagnostic standards of viral culture, indirect immunofluorescence (IFA), and RT-PCR where appropriate. Testing of nasopharyngeal aspirates (NPA) from children, throat swabs, ...
Cincinnati, OH], the ProSpecT Shiga toxin STEC Microplate Assay [Remel Inc., Lenexa, KS], and the Ridascreen Verotoxin Enzyme Immunoassay [r-Biopharm AG, Darmstadt, Germany]) were not significantly different from one another (>0.9) and showed high agreement with PCR for shiga toxin genes. ...
The specificity of the three enzyme immunoassays (Premier EHEC test [Meridian Bioscience Inc., Cincinnati, OH], the ProSpecT Shiga toxin E. coli STEC Microplate Assay [Remel Inc., Lenexa, KS], and the Ridascreen Verotoxin Enzyme Immunoassay [r-Biopharm AG, Darmstadt, Germany]) were not ...