Evaluation of UPlink-RSV: prototype rapid antigen test for detection of respiratory syncytial virus infection. Ann N Y Acad Sci. 2007;1098:476–85. Article CAS PubMed Google Scholar Corstjens P, van Hooij A, Tjon Kon EM, van den Eeden SJF, Wilson L, Geluk A. Field-friendly test ...
Contemporary workflows for antibody discovery commonly uti- lize synthetic selections or the isolation of single B cell clones from convalescent patients or animals to go from >108 sequences to a pool of 103 to 104 candidates targeting the desired antigen. However, once this pool of candidates is...
Medical Test Drug Cup OPI Opiate Rapid Test Panel Kit US$0.20-2.00 / Piece HMPV Antigen Test Cassette Human Metapneumovirus Card US$0.70-0.90 / Piece Latest Human Metapneumovirus Antigen Diagnostic Rapid Test Kit US$0.30-0.50 / Piece Quick Test ...
Primary antibodies (rabbit α–von Willebrand factor [vWF] [Dako, Agilent, Santa Clara, California]; rabbit α–proliferating cell nuclear antigen [PCNA] [Abcam, Cambridge, United Kingdom]; rabbit α-CD34 [Abcam]; rabbit α-CD68 [Abcam]; rabbit α-vimentin [Abcam]; rabbit α-caspase [Cell...
Label-free detection of SARS-CoV-2 Spike S1 antigen triggered by electroactive gold nanoparticles on antibody coated fluorine-doped tin oxide (FTO) electrode Anal. Chim. Acta, 1188 (2021) 339207-339207 Google Scholar [18] Y. Xu, B. Chen, M. He, B. Hu A homogeneous nucleic acid assay ...
Point-of-care tests are the most desirable alternative to ramp up large-scale population screening. Currently, hundreds of initiatives are on course to deliver serologic and antigen detection platforms. Serologic immunoassays targeting IgA, IgM, or IgG provide historic information about viral exposure,...
A diagnostic test for malaria with a 10- or 100-fold improvement in limit of detection (LOD) over microscopy or current RDTs that is simple to use and can be used at the point-of-care could be a useful tool for elimination strategies. RDTs targeting the P. falciparum-specific antigen ...
Staining with PKGI or PKGII primary antibodies was eliminated with preabsorption against their respective antigens (Fig. 1B, middle panel; also see Additional File 1). Staining was also absent in cells treated with fluorescent secondary antibodies alone and in the case of PKGI, when cells ...
After washing in PBS, antigen re- trieval was carried out in 10 mM citrate buffer at 37 °C for 10 min, and followed by 3 washes in PBS. The samples were blocked with 10% goat serum in 1% BSA/PBS for 1 h at room temperature. Primary antibody against GFP (1:50; Santa Cruz ...
The specificity test for PSA (10 ng/mL) was also carried out against alpha-fetoprotein (AFP, 100 ng/mL) and carcinoembryonic antigen (CEA, 100 ng/mL). As shown in Fig. 3c, it was obvious that the signal was as low as that of the blank compared to the higher concentrations of...