英文名Rapid Animal Genomic DNA Isolation Kit 相关类别基因组DNA分离储存常温(10-30℃) 编 号包装库存目录价(￥)您的价格(￥)数量 B518221-005050 PREPS现货230230 B518221-0100100 PREPS现货420420 产品描述 概述 本试剂盒是生工生物研制成功的一种动物基因组 DNA 快速抽提试剂盒。通过含有去污剂的裂解液在高温...
DNAisolation techniquesA simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol ...
A simple, rapid method for isolation of high quality genomic DNA from animal tissues 来自 Semantic Scholar 喜欢 0 阅读量: 5 作者:Q Wu,M Chen,M Buchwald,RA Phillips 摘要: Many studies require isolation of genomic DNA from a large number of animals, e.g., linkage mapping, analysis of ...
The success of the reaction and potential genomic contamination was assessed by PCR amplification followed by gel electrophoresis of the housekeeping gene GAPDH using a 1:5 working dilution of the cDNA. All animals were used under protocols approved by UCL Animal Welfare and Ethical Review Body (...
example, while sex-linked hybrid sterility generally evolves much more rapidly than inviability, both are thought to accumulate more slowly than other forms of ecological or behavioural reproductive isolation2,8. However, most speciation research has focused on animal systems with well-established and ...
Though multicolour labelling methods allow the routine detection of a wide range of fluorescent (immuno)probe types in molecular cytogenetics, combined applications for the simultaneous in situ detection of proteins and nucleic acids are still sporadic i
While our ability to observe the dynamics of developmental processes has greatly advanced, tools to perturb the proteins underlying these processes at the required temporal resolution and efficiency are lacking in animal models. Here, we present iLEXY, which can fill this gap for nuclear proteins. ...
The DNA library was prepared using the Genomic DNA Sequencing Kit SQK-MAP005 according the manufacturer’s protocol (Version MN005_1115_revC_26Nov2014), with small modifications. Without undergoing any shearing process, 2 μg of DNA were treated with PreCR Repair Mix (New England BioLabs, ...
we performed systemic infection and fungal burden assays using a mouse infection model using a selection of WT yeast-form and evolved mutant aggregative strains. To avoid blood vessel blockage and rapid animal death caused byC. aurisaggregates, we convertedC. aurisaggregative cells to single cells ...
Doyle JJ, Doyle JL: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 1987, 19: 11-15. Google Scholar Emshwiller E, Doyle JJ: Chloroplast-expressed glutamine synthetase (ncpGS): Potential utility for phylogenetic studies with an example from Oxalis (Ox...