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内参为actin基因,鉴定actin基因的引物为5’-gcaccctgttcttcttaccga-3’和5’-agtaaggtcacgtccagcaagg-3’。部分实验结果见图1。结果表明,ibrap2-12基因为拟南芥外源基因,在野生型拟南芥中几乎没有表达,但ibrap2-12基因在8个t3代纯合拟转ibrap2-12基因拟南芥的株系中都有不同程度的表达。因此,8个t3代纯合...
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Radioresistance limits the efficacy of radiotherapy against breast cancer, especially the most lethal subtype of breast cancer, triple-negative breast cancer (TNBC). Epithelial-to-mesenchymal transition (EMT) is closely related to tumor radioresistance.
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subclones were isolated for each cell line and then sequenced with the use of 4 kinds of primers as follows: 5′-aggcacggaggaaagtcc-3′(6580/Notch1), 5′-agcagcctctccaccaatac-3′ (7104/Notch1), 5′-taatacgactcactataggg-3′ (T7/pGEM), and 5′-cgccaagctatttaggtgac-3′ (RP/...