Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line. BMC Mol Biol 2010; 11:58.Yan BW, Li DF, Gou KM (2010) Homologous illegitimate random integration of foreign DNA into the X chromo- some of a transgenic mouse line. BMC Mol Biol 11...
Random integrationTransgenicLittle is known about how foreign DNA is randomly integrated into chromosomes in transgenic animals. In the current study, the insertion sites of 36 transgenic mice were mapped by thermal asymmetric interlaced PCR, and 38 junction sequences were obtained from 30 samples. ...
integration. Background DirectmicroinjectionofforeignDNAintothepronu- cleusoffertilisedzygotesisaconventionalmethodto generatetransgenicanimals,whereastheexactintegra- tionsiteandthenumberofcopiesofthetransgeneare randomandunpredictable[1,2].Previousstudiesinvol- ...
总之,以上工作开发了一种高效的基因编辑方法,可以实现随机序列定点整合进入哺乳动物细胞基因组,在基础研究领域有很好的应用前景,例如原位基因进化、原位PAM Library构建、增强子筛选和 DNA barcoding等。 参考文献: [1] Y Jiao, L Zhou, R...
DNA repair, substantially contributes to random integration, and that cells lacking both DNA polymerase y and DNA ligase IV, which is essential for non-homologous end joining (NHEJ), exhibit 100% efficiency of spontaneous gene targeting by virtue of undetectable levels of random integration. Thus...
We have studied the relationship between immortalization of SV40-transformed human embryonic fibroblasts and their SV40 integration sites. From several independently transformed cell pools, we have isolated clones which do not harbor unintegrated SV40 DNA. We have analysed whole-cell DNA from these clone...
Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells 1998, EMBO Journal Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death 1998, EMBO Journal...
此分离柱可用于在DNA随机引物标记和DNA末端标记后去除过量的放射性标记的dNTPs。 12. random的解释 12. With the artificial bogie crawl waves as vibrating resource, the random analysis results of the system space vibration can be obtained. 分别以构架实测蛇行波及前苏联规律性的竖向不平顺函数为横向及竖向...
Statistical analyses of these data suggest that the distribution of integration sites may not be uniform, and the sequence of proviral orientations is not random. On the basis of these results and the observed clustering of orientations, it was postulated that if a DNA sequence was involved in...
45 Single-molecule insights for DNA_RNA_protein interactions and drug discovery and 1:01:06 Skeleta for Monomial Quiver Relations 1:06:54 Solving clustering problems via new swarm intelligent algorithms 42:45 Statistical Network Models for Integrating Functional Connectivity with sMRI and 59:08 The ...