in CIP 2 h sample. Gene count: the number of DEGs enriched in a GO term. Gene ratio: the number of observed DEGs divided by the number of expected genes from each GO term. The cutoff value of thep-value of GO enrichment was 0.05 and the cutoff value of theq-value of GO enrichme...
Briefly, Poly(A)+ mRNA from extracted total RNA were converted to cDNA decorated with universal adapters, sample-specific barcodes and unique molecular identifiers (UMIs) using a template-switching reverse transcriptase. Decorated cDNA from multiple samples were then pooled, amplified (10 PCR cycles...
Screening ofM.hoministransformants by PCR Thetet(M) PCR mixture (50 µL final volume) contained 1X PCR buffer (Promega), 3 mM MgCl2, 0.2 mM dNTPs, 1 μM of each primers (IntMtet1 and IntMtet2), 1 U of HotStart G2 DNA polymerase (Promega), and 5 μL of DNA te...
All the MR brain images were aligned along the line that passes through the anterior and posterior commissures of the brain, corrected for their bias field, and finally spatially normalized to the MNI152 template space using affine transformation35. Atlas selection and registration To reduce the co...