Carney, et al., Random rapid amplification of cDNA ends (RRACE) allows for cloning of multiple novel human cDNA fragments containing (CAG)n repeats, Gene, 155:289, 1995.Carney, et al., Random rapid amplification of cDNA ends ( RRACE ) allows for cloning of multiple novel human cDNA ...
Ozawa 等通过5’ RACE (5’ rapid amplification of cDNA ends) 方法成功扩增出抗体基因编码框5’端的完整序列,该方法通过针对不同同种型抗体恒定区保守序列的特异性反向引物序列GSP 引物(Gene-Specific Primer)混合物, 直接从细胞中RT-PCR 合成cDNA 第一链,对第一链加多聚G尾后(通过末端脱氧核苷酸转移酶TdT实...
T7 RNA polymerase-based linear amplification relies on attaching a T7 promoter sequence to the oligo(dT) primer used for synthesis of the first cDNA strand. After second-strand cDNA synthesis, amplified RNA (aRNA) is generated using T7 RNA polymerase and the double-stranded cDNA as targets ...
Prior work has shown that UMI counting is the only type of amplification correction that improves statistical properties of the data, including variance, power and false discovery rate15. These are important reasons why UMI-based DGE is a method of choice for single cell mRNA sequencing16, where...
Subsequently, the self-ligated DNA was precipitated with ethanol and used as a template for PCR amplification. PCR reactions were performed with Tks Gflex DNA Polymerase (Takara Bio) or EmeraldAmp PCR Master Mix (Takara Bio). Primers used to amplify 50-junctions were as follows: iPCR-EGFP ...
RNA amplification techniques and improved labeling methods have recently been described. These new procedures and reagents allow the use of less input RNA, but they are relatively time-consuming and expensive. Here we introduce a technique for preparing fluorescent probes that can be used to label ...