Problem to be solved: to realize a microscopic Raman spectrometer having a high analytical accuracy for a sample having an uneven surface.\nMicro Raman spectrometerLight transmitting system for forming a beam spot of illumination light on a sampleAmong scattered light from a beam spot formed on a...
A handheld Raman spectrometer was used to detect and identify fungal diseases in wheat, maize, and sorghum seeds (Egging et al., 2018; Farber & Kurouski, 2018). Initially, Raman spectroscopy was not as popular as FTIR due to problems such as sample degradation and fluorescence. During ...
Automated Raman spectrometer The schematic of the home-built Raman microscope is shown in Fig. 1. Here, the ×10 objective was underfilled to reach an illumination diameter of ~8 µm. A motorized stage was installed to automatically park on cells identified through bright field. The entire...
To illustrate the ability to distinguish between different polymorphic structures, three different forms of carbamazepine (a common drug used as an anticonvulsant) were measured with the low frequency Raman spectrometer (Figure 3). The region from 20 - 60 cm-1 shows a clear distinction ...
aSchematic diagram of a Raman spectrometer adapted from Butler et al.20(Permission obtained from Nature Publishing Group). Excitation light is represented by ablue line, while thegreen linerepresents scattered light. Excitation light travels from the laser source, through narrow band pass filters, bea...
The back-scattered Raman radiation is collected by the same microscope objective, partially transmitted by the beamsplitter, and directed into the entrance slit of the Raman spectrometer by means of coupling optics. The accurate location of an aperture, D2, at the focal point improves the spatial...
The Raman microscope focuses the laser to a spot on the sample. We place the sample on a motorised stage that moves the sample under the laser. The spectrometer collects spectra from an array of points across the sample. Fast versions of point-focus imaging are Renishaw's StreamHR™ and ...
Plots were made using Origin 2017. For rapid detection in the field, a portable Raman spectrometer (BWS465-iRman; B &W-Tek, USA) with a 785 nm excitation laser was used with a laser power of 30 mW and an integration time of 2 s. The Raman spectral data were saved as a csv file....
Optical diagram of an FT-Raman spectrometer. The heart of the instrument is the interferometer head, consisting of the beam splitter and fixed and moving mirrors. Table 2-6. Advantages and Disadvantages of FT-Raman AdvantagesDisadvantages Reduction or elimination of fluorescence High resolution High ...
The resonance Raman spectrometer and detectors are the same as for the conventional or multichannel Raman systems. The most obvious advantage of resonance Raman spectroscopy is the enhancement of the observed UV excitation by 6 to 7 orders of magnitude. These high intensity enhancements allow trace ...