Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to assess the chemical composition of individual live microorganisms in near real time. In this Primer, we outline the methodology and potential for its application to microbiology. We describe the technical aspects of ...
Raman microspectroscopy for microbiology. Nat Rev Methods Primers. 2021a;1:80. 58. Yuan X, Song Y, Song Y, Xu J, Wu Y, Glidle A, et al. Effect of laser irradiation on cell function and its implications in Raman spectroscopy. Appl Environ Micro- biol. 2018;84. 59. Lee KS, Palati...
Single cell stable isotope probing in microbiology using Raman microspectroscopy Using single cell Raman microspectroscopy, biochemical fingerprints of individual microbial cells can be obtained in an externally label-free and non-... Y Wang,WE Huang,L Cui,... - 《Curr Opin Biotechnol》 被引量: ...
Candida tropicalis Confocal Raman microspectroscopy 400–1800 Maquelin K. et al. (2002) Development of a rapid and accurate identification method for clinically relevant Candida species from 6-h microcolonies on a solid culture medium. The data obtained was analyzed 2003 Candida albicans, Candida ...
T. elongatais one of the few known PAO that exist in pure culture and it was thus selected to test for qualitative and quantitative assessment of its intracellular storage polymers using Raman microspectroscopy. However, it should be noted thatT. elongatais closely related but not identical toTet...
the Apollo enables scientists to measure the Raman spectra from microscopic samples or microscope sampling areas of large samples. It can be added to the company’s microspectrophotometer, offering Raman microspectroscopy to UV-visible-NIR absorbance, reflectance and fluorescence microspectroscopy and imagin...
A fundamental goal in the life sciences is to understand how the elemental building blocks (nucleobases, amino acids, etc.) are composed in living org
Single cell stable isotope probing in microbiology using Raman microspectroscopy. Curr Opin Biotechnol. 2016;41:34–42. Article CAS Google Scholar Huang WE, Griffiths RI, Thompson IP, Bailey MJ, Whiteley AS. Raman microscopic analysis of single microbial cells. Anal Chem. 2004;76(15):4452–8...
Raman microspectroscopy, being able to look at molecular vibrations in any form of microscopic samples in a nondestructive manner, provides a promising tool for in vivo metabolic imaging when coupled with the SIP method. In addition to the nondestructive character, it has high chemical specificity an...
Single cell stable isotope probing in microbiology using Raman microspectroscopy. Curr. Opin. Biotechnol. 41, 34–42 (2016). Article CAS PubMed Google Scholar Haider, S. et al. Raman microspectroscopy reveals long-term extracellular activity of chlamydiae. Mol. Microbiol 77, 687–700 (2010). ...