*使用本品系发表的文献需注明: Rag1-KO(Rag1-EGFP) mice (Cat. NO. NM-KI-00069) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 构建策略 图1 Rag1构建策略图。 Rag1、Rag2基因敲除小鼠表达验证 图2 4种小鼠外周血功能T、B淋巴细胞含量流式细胞术检测结果。
肿瘤组织HE染色病理切片结果显示Rag1 KO小鼠和Nude小鼠肿瘤切片较为相似。该品系小鼠具有替代Nude,NOD-SCID小鼠作为肿瘤成瘤模型的潜力。 应用领域:免疫缺陷,荷瘤模型 *使用本品系发表的文献需注明: Rag1-KO(Rag1-EGFP) mice (Cat. NO. NM-KI-00069) were purchased from Shanghai Model Organisms Center, ...
Rag1-KO小鼠(C57BL/6j背景)。 定义:RAG=Recombination-Activating Gene(重组激活基因),包含两个基因,rag 1和rag 2;最终翻译产物是Rag 1 和Rag 2两个蛋白质。 作用:二者在T细胞和B细胞成熟过程中形成四聚物,负责TCR和BCR的RSS序列的识别,从而影响到VDJ重排。简言之,没有它们就没有成熟的T细胞和B细胞。Rag ...
2001. Early death and severe lymphopenia caused by ubiquitous expression of the Rag1 and Rag2 genes in mice. Eur J Immunol 31:376376772.Barreto V, Marques R, Demengeot J. Early death and severe lymphopenia caused by ubiquitous expression of the Rag1 and Rag2 genes in mice. Eur J ...
Rag1 knockout (KO) mice are essential for studying immune system deficiencies, as they lack mature T and B cells. By disrupting V(D)J recombination via the Rag1 gene, these models provide insights into T and B cell development, enabling research on immun
3.rag缺陷型scid患者在rag1或rag2中存在突变,这是t细胞受体(tcr)和b细胞受体(bcr)基因组装所必需的。患病儿童通常会经历各种严重且危及生命的感染,包括肺炎、脑膜炎和败血症。通过同种异体干细胞移植(allo‑sct),采用健康的未修饰同种异体干细胞替换患病骨髓是目前rag‑scid的唯一疗法。尽管匹配sct受者的总生存...
(RAG1) andRAG2, which together facilitate V(D)J recombination of germline TCR loci14, was ablated via CRISPR–Cas9 gene editing in PSCs. To generate the class I MHC-null phenotype, β2-microglobulin (B2M) was targeted for knockout (KO)15,16. Full T cell differentiation, including ...
During V(D)J recombination, RAG1 and RAG2 cleave DNA adjacent to highly conserved recombination signals, but nothing is known about the protein–DNA complexes that exist after cleavage. Using a properly regulated in vitro V(D)J cleavage system, together with nuclease sensitivity, mobility shift,...
Abl-transformed mouse pre-B cell lines generated from wild-type (WT) and RAG2−/− mice carrying an Eμ-Bcl2 transgene were kindly provided by Dr Craig Bassing (University of Pennsylvania School of Medicine, Philadelphia, PA). The human BCR-ABL-positive B-ALL cell lines BV173 and SUP...
More importantly, the mouse Sirpa gene is genetically linked with the Rag1 and Rag2 genes, making it extremely difficult to derive NRG-Fah mutant mice with the NOD Sirpa allele by genetic crossing. The ability to introduce additional mutations directly in embryos from NRG or NSG mice would ...