2.2.4 Notes • Homogenization and sample preparation have to be performed at 4°C using precooled buffers. • Avoid foam formation as well as possible. • Be aware that ingredients of the RIPA solubilization buffer may interfere with protein determination assays. 2.3 TCA Precipitation of Prot...
B., Dudka, L., Richardson, S., Immunobiology of primary intracranial tumors: Part I: Studies of the cellular and humoral general immunocompetence of brain tumor patients. J. Neurosurg.46 (1977), 467–476. PubMed Google Scholar Mahaley, M. S., Steinbok, P., Aronin, P., Dudka, L...
Pre-targeting via trans-cyclooctene-decorated dendritic scaffold-modified huA33 immunoconjugate produced significantly high tumor uptake, and a dextran-based masking agent modified with tetrazine dramatically decreased the absorbed dose in the blood. Unfortunately, the addition of the masking agent resulted...
Furthermore, NF-κB protein nuclear translocation was tested by immunofluorescent staining, and the nuclear staining intensity of p-NF-κB was decreased by NPs-TPP-NIT treatment (Fig. 5N). The current findings indicated that NPs-TPP-NIT inhibited IR-induced splenic inflammatory injury in mice by...
上海玉博生物科技有限公司 主要致力于“CytoTox 96® Non-Radioactive Cytotoxicity Assay,非放射性细胞毒性检测试剂盒 promage G1780”的生产销售。多年的“CytoTox 96® Non-Radioactive Cytotoxicity Assay,非放射性细胞毒性检测试剂盒 promage G1780”生产与销售
Solid tumors are often riddled with hypoxic areas, which develops as a result of high proliferation. Cancer cells willingly adapt and thrive in hypoxia by activating complex changes which contributes to survival and enhanced resistance to treatments, suc
c, Summary of kinetic constants according to SPR assay. d, Representative confocal fluorescence images of visualizing the cellular efflux of FAPI-mFS-AF488 probe (6) and its FS-hydrolysed control (7). The HT-1080-FAP cells were pre-treated with fluorescent probes 6 or 7 (10 μM in ...
I The half-life of G6PD protein in cells treated with either CHX alone, CHX + GSK2126458, or CHX + GSK2126458 + CQ was detected by immunoblotting. J The interaction between HSPA8/HSC70 and G6PD in SBC2 cells were validated by Co-IP assay. Full size image Protein ...
Effects of X-rays on DNA damage, gene expression, cell cycle and viability in H460, Calu-1 and A549 cells.ARepresentative confocal immunofluorescent images of γ–H2AX foci at 0 Gy and 8 Gy, 30 min after IR.BDetection of γ–H2AX foci in normoxia and hypoxia at 30 min and 24 h pos...
Five-micron-thick sections were obtained from formalin-fixed, paraffin-embedded tissues and set aside for p53 antibody (DO7) and PD-L1 (SP263) assay using a VENTANA OptiView DAB universal kit (Roche, Bazel, Switzerland) and VENTANA BenchMark ULTRA automated slide stainer (Roche, Bazel, ...