We characterized a novel gene, previously a predicted open reading frame for hypothetical protein FJ1 1164, which falls within this chromosomal interval. The gene for FLJ1164 is highly conserved in vertebrates and encodes a protein with iron-sulfur cluster and S-adenosyl methionine (SAM) binding ...
The HExxH domain is a defining feature of zinc metalloproteases. Yet the HExxH motif-containing domain studied here catalyses β-hydroxylation and is an α-ketoglutarate non-haem iron enzyme. We determined the crystal structure for this HExxH protein at 2.8 Å, unveiling a distinct ...
A third system pairs a radical SAM domain peptide maturase with selenocysteine-containing targets, suggesting a new biological role for selenium. These and several additional novel maturases that cooccur with predicted target peptides share a C-terminal additional 4Fe4S-binding domain with PqqE, ...
SAM S-adenosyl-l-methionine SPASM domain subtilosin A, pyrroloquinoline quinone, anaerobic sulfatase, and mycofactocin domain RiPP ribosomally synthesized and posttranslationally modified peptide sactipeptide sulfur-to-α-carbon thioether–containing peptide Rum Ruminococcin RRE RiPP precursor peptide recog...
The TIM barrel domain is colored in white. The Twitch/SPASM domains are colored by protein. The AuxI and II clusters are shown as yellow and orange sticks. Extended Data Fig. 3 EPR, UV-visible and HYSCORE analysis of EpeE in absence or presence of SAM. a, Temperature dependence of EPR...
Viperin is an iron-sulfur protein that inhibits genome synthesis of tick-borne encephalitis virus via radical SAM domain activity. Cell Microbiol. 2014;16(6):834-48. doi:10.1111/cmi.12241.Viperin is an iron‐sulfur protein that inhibits genome synthesis of tick‐borne encephalitis virus via ...
These conserved domains correspond to the superfamily denoted Elongator protein 3/MiaB/radical SAM, which contains several radical SAM enzyme families, the protein family radical SAM MSMEG_0568, which has no characterized function, the radical SAM superfamily, and a domain found in biotin synthase ...
Fig.5b), suggesting that our protocol gives rise to incomplete reconstitution and possibly a fraction of [3Fe–4S] clusters.26IseH exhibited a typical UV-Vis spectrum for a [4Fe–4S] cluster-containing protein (Supplementary Fig.6). Like other radical SAM enzymes, IseH cleaved SAM to form...
Iron-Sulfur Protein Post-translational Modification (PTM) Radical S-Adenosylmethionine (SAM) Structural Biology AdoMet Sorry, something went wrong. Please try again and make sure cookies are enabledCited by (0)* This work was supported by an MIT Office of the Dean of Graduate Education Fellowship...
iron–sulfur protein molecular cell biology sulfur Cia targeting complex biogenesis metal biology viperin RSAD2 (radical S-adenosylmethionine domain containing 2) is one of the interferon (IFN)-stimulated genes that is strongly up-regulated by type I IFNs, lipopolysaccharide, polyinosinic/polycytidylic ...