Radioembolization is a technique used for liver cancer in which radioactive beads called microspheres are injected into the artery feeding the tumor (ACS 2014a; Kishore 2017). Brachytherapy implants may stay in place for minutes to days, or in some cases, permanently. The intensity of the ...
The immunoprecipitated protein with beads were boiled in SDS sample buffer, and the supernatants were analyzed on SDS-polyacrylamide gel and subjected to Western blot analysis using anti-Rb antibody or anti-MDM2 antibody. RESULTS Ionizing Radiation Induces EGR-1 Protein That Transactivates via the GC...
mRECIST and EASL responses at early time point by contrast-enhanced dynamic MRI predict survival in patients with unresectable hepatocellular carcinoma (HCC) treated by doxorubicin drug-eluting beads transarterial chemoembolization (DEB TACE). Ann Oncol. 2013;24(4):965–73. Article PubMed CAS Google...
Mouse 38-plex kits were purchased form Affymetrix and used according to manufacturer’s recommendations. The Stanford Human Immune Monitoring Core processed samples as follows. Antibody linked beads were added to a 96-well plate washed with Biotech Elx405 washer and 60 μl of concentrated conditi...
Proteins were eluted by boiling the beads for 5 min in 100 μL SDS sample buffer with β-mercaptoethanol. 30 μL were separated by SDS gel electrophoresis. Results To analyze cap-dependent protein translation in response to IR, Jurkat T lymphoma cells were irradiated with 10 Gy. At indicated...
Pulmonary endothelial cells were isolated by magnetic beads coated with antibody (rat anti-mouse) to PECAM-1 (BD Pharmin- gen), and seeded for subculture (passage 1). At approximately passage 2, cells were incubated with fluorescently labelled diacetylated LDL followed by FACS sorting for further...
(Thermo Fisher Scientific) for 4 h at 4 °C with agitation. The beads were harvested and sequentially washed on a magnetic stand with 1 ml each of the following buffers: low-salt immune complex wash buffer (0.1% SDS, 1% Triton, 2 mM EDTA, 20 mM Tris [pH 8.0], 15 mM...
Beads were washed three times with lysis buffer and eluted by boiling in SDS sample buffer. Immunoprecipitates were analyzed by immunoblotting using the indicated antibodies. Large scale IPs were performed on EGFRvIII-expressing astrocytes using the same protocol and samples were submitted for LC-MS/...
Transarterial chemoembolization with drug-eluting beads versus stereotactic body radiation therapy for hepatocellular carcinoma: Outcomes from a multicenter, randomized, phase 2 trial (the TRENDY trial) Int J Radiat Oncol Biol Phys (2023)View more references ...
(FIG. 11A) BMDCs were cultured with MC38 tumor cells that were pretreated with 0.2 μM or 1 μM 6-thio-dG for 6 hrs, and then DCs were purified with magnetic beads and subjected to western blot. (FIG. 11B) BMDCs from wild-type (WT) or Tmem173KO mice were cultured with MC38 ...