Full size image Voltage-gated sodium (Na+) channels form heteromeric proteins consisting of α subunits formed by macropores and small auxiliary β subunits. NaV1.5 is encoded by the SCN5A gene, which encodes the α subunit. According to research reports, SCN5A mutations can cause a variety o...
Co-transfection of the pGL3-CNS2 luciferase reporter construct with a plasmid encoding the N-terminal truncated mutant of Tiam1 (C1199-Tiam1), a Tiam1 variant that has been shown to be more stable and more active than the full length37, resulted in a significant amplification of RORgt-...
Full size image Docetaxel, doxorubicin, and cisplatin are the first line agents of breast cancer chemotherapy in the neoadjuvant and adjuvant setting9. To test whether Rac1confered chemoresistance in breast cancer, we treated MD-MBA-231 cells with the above chemotherapeutic agents after Rac1 silenci...
Download: Download full-size image Figure S3. Generation of the Targeted Chn2 Deletion Allele, Related to Figures 3 and 4 (A) Chn2 gene targeting strategy. Deletion of coding exon 2 (bp 50-88 of the mouse β2-Chimaerin coding sequence, corresponding to aa 17–30) was performed by cross...
Download: Download full-size image Figure 1. The interaction of Rho, Rac, and Cdc42 with plexin-B1. Yeast strains containing the Rho GTPases in the integrated bait vector pYTH9 were transformed with the pACTII prey vector containing the plexin-B1 C terminus (amino acids 1724–2135) and ...
Because writing full sentences and paragraphs by hand is time-consuming (plus there’s that whole legibility thing), I dictate everything. Ditto for my partners. Our coding and compliance departments like this and we feel well-covered should an audit occur. ...
1. Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.2. Department of Clinical Laboratory, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong ...
Full size image Tiam1 deficiency partially reduces IL-17A expression and EAE Given the induction of Tiam1/Rac1 in Th17 cells, we investigated the role of the GEF, Tiam1, in IL-17 expression. Naive CD4+ T cells purified from splenocytes of Tiam1−/− mice and their control WT litt...
Indeed, when we reintroduced full- length and mutant murine RIN2 constructs in human ECs in which endogenous hRIN2 had been simultaneously silenced, we observed that both EC adhesion to ECM proteins and Rac1 GTP loading depend on the RH/VPS9 and RA domains of RIN2 that bind to the ...
A full-length Gα12 cDNA was isolated form a rat brain λZAPII cDNA library (Stratagene) by hybridization screening using the 219-bp PCR fragment as a probe under high stringency condition (at 42°C in the presence of 50% formamide and 0.9 M NaCl). The nucleotide sequence of the cloned...