False discovery rate (FDR) was calculated by searching the data against the corresponding reverse database. Peptides with 1% FDR were used to identify proteins. Proteins identified with only one peptide identification from Spectrum Mill or Mascot was further validated by manual inspection of MS/MS ...
In all search programs in the suite the gapped alignment routines are integral to the database search itself. Gapping can be turned off if desired. The default penalty (Q) for a gap of length one is Q=9 for proteins and BLASTP, and Q=10 for BLASTN, but may be changed to any inte...
(Fig.1c, Table2). Analysis of 1412 unique full-length RABV G amino acid sequences from the database indicated that positions 190, 194 and 231 display the highest variability across strains, with L231 making only minor contributions to the BSA (Fig.1c). The most frequent polymorphisms at ...
Hits that affected RABV at 18 h (yellow) or 36 h (red) or both (orange) are indicated (see link on the KEGG database). Displayed proteins and enzymatic functions correspond to identified genes as follows: 2.7.1.52 = FUK, 3.1.3.11 = FBP2, and 3.1.3.46 = PFKBP1/...
Database for Annotation, Visualization and Integrated Discovery SD: Standard deviation.References Ray NB, Ewalt LC, Lodmell DL: Rabies virus replication in primary murine bone marrow macrophages and in human and murine macrophage-like cell lines: implications for viral persistence. J Virol 1995, 69...
The GeneBank database was searched using the amino acid sequence of the RABV HEP-Flury P protein. The one-hundred most similar sequences were aligned and analyzed with BioEdit v.7 software [44]. Phylogenetic analysis was performed using MEGA11 software [45]. Supplementary Materials The following...
The following data were entered into the WS National Rabies Management Program (NRMP) database: date and GPS coordinates of sample collection; species; sex; relative age (juvenile or adult); and reproductive status. Samples were categorized for data analysis as detected, submitted, and tested. ...