False discovery rate (FDR) was calculated by searching the data against the corresponding reverse database. Peptides with 1% FDR were used to identify proteins. Proteins identified with only one peptide identification from Spectrum Mill or Mascot was further validated by manual inspection of MS/MS ...
The MS/MS signals were then processed against the Uniprot human protein database (155,558 sequences as of October 15, 2016) using the Mascot algorithm (version 2.2; Matrix Science) with the following parameters: dynamical modifications: oxidation (M); fixed modifications: carbamidomethyl: (C); ...
False discovery rate (FDR) was calculated by searching the data against the corresponding reverse database. Peptides with 1% FDR were used to identify proteins. Proteins identified with only one peptide identification from Spectrum Mill or Mascot was further validated by manual inspection of MS/MS ...
In all search programs in the suite the gapped alignment routines are integral to the database search itself. Gapping can be turned off if desired. The default penalty (Q) for a gap of length one is Q=9 for proteins and BLASTP, and Q=10 for BLASTN, but may be changed to any inte...
Database for Annotation, Visualization and Integrated Discovery SD: Standard deviation.References Ray NB, Ewalt LC, Lodmell DL: Rabies virus replication in primary murine bone marrow macrophages and in human and murine macrophage-like cell lines: implications for viral persistence. J Virol 1995, 69...
RABV epitopes within L, M, and P amino acid sequences were identified using resources from the IEDB-AR (immune epitope database) [42], accessible through the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) [43]. 2.13. RABV Isolation and Neutralization with mAb A6 ...
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