Significance:Thesefindingsshowthatendosomal/lysosomalRAB5andRAB7,whichregulatemitophagy,areessentialforthesurvivalofcoloncancerstemcells. 鉴于癌症干细胞(CSC)在耐药性和复发中的关键作用,靶向CSC在癌症治疗中仍然具有前景。在这里,我们显示参与内溶酶体途径的RAB5/7通过调节线粒体途径在维持CSC存活中起关键作用。...
TBC-2是人类TBC1D2A和TBC1D2B的线虫同源物 [9]。TBC1D2A具有Rab7-GAP活性,与LC3和LRRK1相互作用以调节自噬[10, 11],TBC1D2B与Rab22之间存在相互作用,但其细胞功能有待进一步阐明[12]。 与哺乳动物同源物不同,TBC-2在内吞体...
Rab5‐mediated endosome–endosome fusion regulates hemoglobin endocytosis in Leishmania donovani . EMBO J. 2003; 22 (21):5712–5722. doi: 10.1093/emboj/cdg557. [ Cross Ref ]S. B. Singh, R. Tandon, G. Krishnamurthy et al., "Rab5-mediat- ed endosome-endosome fusion regulates hemoglobin ...
7a,b). In addition, knockdown of the late endosome protein Rab7 led to increased FCCP-mediated cell death in WT and Atg5−/− MEFs (Fig. 7c–e). Rab5 exists as three isoforms with overlapping functions and all three isoforms are expressed in MEFs (Supplementary Fig. 10a,b). The ...
On the other hand, IL-12 induced Rab7 expression through p38. Another study showed that activation of p38 MAPK regulates endocytosis by regulating the activity of Rab5 accessory proteins such as Rab5-GDI, EEA1, and rabenosyn-5, which are known to regulate membrane transport during endocytosis...
Barbieri MA, Li G, Mayorga LS, Stahl PD (1996) Characterization of Rab 5:Q79L-stimulated endosome fusion. Arch Biochem Biophys 326:64–72 Article CAS PubMed Google Scholar Bucci C, Thomsen P, Nicoziani P, McCarthy J, van Deurs B (2000) Rab7: a key to lysosome biogenesis. Mol Bio...
7a,b). In addition, knockdown of the late endosome protein Rab7 led to increased FCCP-mediated cell death in WT and Atg5−/− MEFs (Fig. 7c–e). Rab5 exists as three isoforms with overlapping functions and all three isoforms are expressed in MEFs (Supplementary Fig. 10a,b). The ...
Aberrant neuronal Rab5-endosome signaling, therefore, drives a pathogenic cascade distinct from β-amyloid-related neurotoxicity, which includes prodromal and neurodegenerative features of AD, and suggests Rab5 overactivation as a potential therapeutic target. Graphical Abstract Download: Download high-res ...
2 A). The addition of Rab7 was unable to restore endosome fusion (data not shown). Moreover, incubation of GDI-depleted membranes with [3H]Rab5 D136N resulted in the accumulation of radiolabeled Rab5 on the membranes (Fig. 2 B). Rab5 D136N binding was temperature- and ATP-dependent ...
Arrest of Mycobacterial Phagosome Maturation Is Caused by a Block in Vesicle Fusion between Stages Controlled by rab5 and rab7 The transformation of phagosomes into phagolysosomes involves gradual acquisition of markers from the endosomal compartment. Members of the rab family of small... LE Via,D...