Do not aliquot the antibody. Protocol Specificity / Sensitivity Rab5 (C8B1) Rabbit mAb detects endogenous levels of total Rab5A, Rab5B and Rab5C protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / P
Briefly, an anti-active Rab5 mouse monoclonal antibody was used to immunoprecipitate the GTP-bound form of Rab5 in the cell extract. Immunoprecipitated Rab5 was then detected by immunoblot analysis using a rabbit anti-Rab5 polyclonal antibody. Immunoblot analysis Total protein extraction from cell ...
B mCherry-ATG5 puncta per cell was quantified and expressed as means ± s.d. of at least 20 cells (**P < 0.01). C Western blotting of ATG12–ATG5 conjugation detecting by antibody against ATG12 in HeLa cells transfected with vector or CMTM7 plasmid. The intensity of the ...
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(1:1000)13, rabbit anti-Rabankyrin-5 (1:1000)47, rabbit anti-Akt (Cat.# 9272, Cell Signaling, Beverly, MA, USA, 1:100 in IF), mouse anti-MAP2 (Cat.# MAB3418, Chemicon Europe, 1:1000 in IF), mouse anti-phosphorylated neurofilament antibody (Cat.# SMI-31, HISS Diagnostics, ...
Rbpn-5 is uniformly expressed and localises to punctate apical structures together with some cytoplasmic staining deeper within the cell (Fig. 3A--C). The punctate staining co-localises well with an antibody against Rab5, indicating that these vesicles are probably early endosomes. As previously ...
Whole cell lysates (WCL) were analyzed utilizing antibody recognizing tubulin. The experiment was repeated three times with similar results. Depletion of Dynamin 2 Blocks Insulin Receptor Internalization but Not Insulin Signaling—A previous study showed that depletion of dynamin 2 decreased the ...
(1:1000)13, rabbit anti-Rabankyrin-5 (1:1000)47, rabbit anti- Akt (Cat.# 9272, Cell Signaling, Beverly, MA, USA, 1:100 in IF), mouse anti-MAP2 (Cat.# MAB3418, Chemicon Europe, 1:1000 in IF), mouse anti-phosphorylated neurofilament antibody (Cat.# SMI-31, HISS Diagnostics, ...
(P), an inactive form of MiTMAB, a negative control for endocytic blockade, does not prevent APPL1wt co-IP with βCTF. In all co-IP experiments, 10% of total cell lysates is used as an input standard. (+) and (−) indicates the presence and absence of the antibody used in IP,...
gingivalis invasion was inhibited by an antibody to ICAM-1 in Ca9-22 cells. Silencing of Rab5 mRNA inhibited P. gingivalis invasion. Furthermore, the JNK inhibitor SP600125 inhibited invasion of P. gingivalis and also decreased the active form of Rab5 in Ca9-22 cells. Conclusion TNF-α ...