网络增强型绿色荧光蛋白基因 网络释义 1. 增强型绿色荧光蛋白基因 ...AV—IFN—α-1b),C组:重组腺相关病毒携带增强型绿色荧光蛋白基因(rAAV-EGFP),D组:磷酸缓冲液;建模5d后B组… www.39kf.com|基于3个网页
AAV transfection:neural stem cells were transfected with virus transfection solution comprising rAAV-2-EGFP and complete culture solution at multiplicity of infection = 10~5.Negative control: physiological saline was used instead of virus transfection solution. MAIN OUTCOME MEASURES:At different time ...
【目的】研究不同血清型腺相关病毒(rAAV)载体介导的增强绿色荧光蛋白基因(EGFP)对体外培养犬骨髓间充质干细胞(MSC)的转染效率及其毒性作用。【方法】应用密度梯度离心法及贴壁筛选法分离培养犬MSC,倒置显微镜及Giemsa染色观察细胞形态,透射电镜观察其超微结构,流式细胞仪鉴定其表面标记物。取第3...
An ideal rAAV gene editing system not only effectively edits genes at specific site, but also prevents the spread of the virus from occurring off-target or
CONCLUSION:rAAV-2-EGFP transfection of neural stem cells was superior to liposome transfection.中国神经再生研究(英文版)Yanqiang Zhan Wei Xiong Jie Xu Bo Yin Furong WangDepartment
CONCLUSION:rAAV-2-EGFP transfection of neural stem cells was superior to liposome transfection.中国神经再生研究(英文版)Yanqiang Zhan Wei Xiong Jie Xu Bo Yin Furong Wang Suming Zhang
目的对表皮干细胞(ESCs)进行分离培养和鉴定,利用携带绿色荧光蛋白(eGFP)基因的重组腺相关病毒(rAAV)转染表皮干细胞,探索不同滴度rAAV2/eGFP的转染效率.方法1.获取胎鼠表皮单细胞悬液.2.利用层黏连蛋白(laminin)和Ⅳ型胶原(CollagenⅣ)筛选ESCs,在体外进行培养和鉴定.3.将ESCs按5×104个/孔接种入24孔培养板中,...
eGFP proteinrAAV-SaCas9 systemAn ideal rAAV gene editing system not only effectively edits genes at specific site, but also prevents the spread of the virus from occurring off-target or carcinogenic risks. This is important for gene editing research at specific site in vivo. We report a ...
rAAV-SaCas9系统肌肉生长抑制素肌肉注射成肌细胞目前,CRISPR/Cas基因编辑技术在培育转基因家畜中已经被广泛应用,但是转基因家畜还存在抗病力弱,组织易癌变及功能紊乱等缺点.因此,基因编辑成年家畜的特定组织成为提升家畜生产性能并且避免转基因动物缺点的一种有效方法.本研究构建了一个rAAV9-SaCas9-eGFP系统,包括由EF1...