哺乳动物细胞中,约有5%的区域会形成R-loop,多数集中在转录起始区和转录终止区,由RNA Pol I,II与III转录的RNA均可与DNA形成R-loop,表明其可来源于几乎所有类型的RNA。R-loop可以在细菌到人类等生物体细胞内频繁的形成,进而影响转录并导致基因组的不稳定性,异常R-loop的形成和积累与许多疾病的发生发展具有密切关系...
相对于其它检测R-loop的方法,MapR具有以下几个特点:1)MapR的整个实验过程是在细胞内,并且利用MNase对核酸序列进行切割,能够检测到一些相对弱的或是瞬时形成的R-loop;2)MapR不依赖S9.6抗体,不需要构建稳定表达RNaseH的细胞系,整个实验过程只需要一天时间,并且适用于不同的细胞类型;3)MapR技术也可以在少量细胞有效...
相对于其它检测R-loop的方法,MapR具有以下几个特点:1)MapR的整个实验过程是在细胞内,并且利用MNase对核酸序列进行切割,能够检测到一些相对弱的或是瞬时形成的R-loop;2)MapR不依赖S9.6抗体,不需要构建稳定表达RNaseH的细胞系,整个实验过程只需要一天时间...
近日,中国农业科学院深圳农业基因组研究所(岭南现代农业科学与技术广东省实验室深圳分中心)徐炜课题组联合清华大学孙前文课题组与孟安明课题组开发了一项高精度、超微量R-loop定量测序技术ULI-ssDRIP-seq,并基于该项技术揭示了R-loop在斑马...
Mutations in genes directly involved in R-loop biology, such as SETX (senataxin), or unstable DNA expansion eliciting R-loop generation, such as C9ORF72 HRE, can cause DNA damage and ultimately result in motor neuron cell death. In this review, we discuss current advancements in this field ...
Through SSB1-mediated recognition of single-stranded DNA, SOSS–INTAC stimulates promoter-proximal termination of transcription and attenuates R-loops associated with paused RNA polymerase II to prevent R-loop-induced genome instability. SOSS–INTAC-dependent attenuation of R-loops is enhanced by the...
In addition, mutations in factors implicated in R-loop biology, such as RNase H and SETX (senataxin), lead to devastating human neurodegenerative disorders, highlighting the importance of correctly regulating the level of R-loops in human cells. In this review we summarise current advances in ...
et al. Human Rad52 promotes XPG-mediated R-loop processing to initiate transcription-associated homologous recombination repair. Cell 175, 558–570.e11 (2018). Article CAS PubMed Google Scholar Cohen, S. et al. Senataxin resolves RNA:DNA hybrids forming at DNA double-strand breaks to prevent...
(type = "Classification", library = "randomForest", loop = NULL) customRF$parameters <- data.frame( parameter = c("mtry", "ntree"), class = rep("numeric", 2), label = c("mtry", "ntree")) customRF$grid <- function(x, y, len = NULL, search = "grid") {} ...
R-loop score for malignant cells correlates with the disease outcome in LUAD The workflow of our study is shown in Figure S2. To explore the relationship between R-loops and LUAD progression, we first integrated two separate single-cell RNA-seq datasets (GSE123904 and GSE131907). As shown ...