5 min quick ligation
4d). Additionally, we developed an efficient protocol along with an improved buffer system (called TNT-Buffer; 50 mM Tris-HCl pH7.5, 2 mM DTT, 10 mM MgCl2, 1 mM ATP and 2% PPG) that allowed EarI and LguI enzymes to work well in combination with T4 DNA ligase in a “one...
Differently, the analogous reactions performed using the regular T4 DNA ligase buffer, the LR Reaction from the Gateway system or the isothermal (Gibson) assembly for cloning 1 element retrieved, respectively, 2.6 × 104 (SE ± 16.3%), 4.7 × 104 (SE ± ...