Proteins were digested in-gel overnight at 37 °C using sequencing grade bovine trypsin (Roche Diagnostics; 10 ng/μl in 50 mm ammonium bicarbonate, pH 8.5). The supernatant was used for analysis by nanoflow liquid chromatography and FT-ICR-MS/MS. On-line Nanoflow Liquid Chromatography FT-...