Derivatization took place in the same cups as follows: 3–4 µl of 25% TMAH in methanol (both from Fisher Scientific), depending on the sample size, were added directly to the sample in each cup with a 50-µL syringe and, after 1 min, loaded onto the autosampler. The interface ...
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采用二倍稀释法测定精油的最小抑菌浓度 (Minimal inhibit concentration, MIC).将精油用 40%乙 醇溶液稀释至浓度为 200 滋g/mL,摇匀备用.取 300 滋L 稀释后的精油于装有 5.70 mL LB 液体培养基的试管 中,然后采用二倍稀释法,依次得到含有浓度分别为 10.00,5.00,2.50,1.25,0.63,0.31,0.16,0.08 滋g/mL 的...