Quantitative real time PCR analysis (qPCR) of marker genes for nitrogen and phosphorus acquisition.Eva NouriFlorence BreuillinSessomsUrs FellerDidier Reinhardt
Quantitative Real-time PCR(qPCR) Analysis of Main Methanogens in Pit Mud of Different Pit Age 来自 Semantic Scholar 喜欢 0 阅读量: 79 作者:Q Luo,C Liu,WU Zhengyun,W Zhang 摘要: Abstact: Methanogens is the important functional bacteria in pit mud for the fermentation of Nong-flavor liquor...
Key Applications of Real-Time PCR: While qPCR has been used for a wide variety of applications over the years, several uses of technique continue to be particularly popular. These include: Gene expression (mRNA) analysis microRNA and non-coding RNA analysis ...
Quantitative Real-Time Polymerase Chain Reaction (qPCR) :实时定量聚合酶链反应(PCR)定量,实时,帮助,聚合酶链,real,time,chain,QPCR,qPCR,定量PCR 文档格式: .pdf 文档大小: 328.54K 文档页数: 12页 顶/踩数: 0/0 收藏人数: 0 评论次数: 0
实时荧光定量PCR 利用聚合酶链式反应(PCR)来定量分析待测样品中特定的DNA序列。如果使用该方法,就可以调查靶基因的表达量和样本中病毒基因的含量。因为一般的qPCR是实时定量分析的,所以很多时候,qPCR又称作实时荧光定量PCR。常用的检测方法有绿色环保法(SYBR Green)和TaqMan探针法(TaqMan Probe)两种。
the expression of the eight selected HKGs of YLS was investigated by RT-qPCR. All genes were expressed and visualized as a single band of the expected sizes on 2% agarose gels. All amplicons were sequenced and displayed 99–100% identity with the corresponding gene sequences. The PCR efficien...
Real-time PCR, or quantitative PCR (QPCR), has been developed to rapidly detect and quantify the total number of yeasts in wine without culturing. Universal yeast primers were designed from the variable D1/D2 domains of the 26S rRNA gene. These primers showed good specificity with all the...
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实时定量PCR(QPCR)。 翻译结果2复制译文编辑译文朗读译文返回顶部 数量实时的 PCR (qPCR)。 翻译结果3复制译文编辑译文朗读译文返回顶部 定量PCR (qPCR)。 翻译结果4复制译文编辑译文朗读译文返回顶部 定量实时pcr(qpcr)。 翻译结果5复制译文编辑译文朗读译文返回顶部 定量实时PCR (qPCR)。 相关内容 a现实中影响国际...
Quantitative real-time polymerase chain reaction (qPCR) is an efficient and widely used technique to monitor gene expression. Housekeeping genes (HKGs) are often empirically selected as the reference genes for data normalization. However, the suitability of HKGs used as the reference genes has been...