The invention relates to a fecal DNA quantitative methylation specific PCR detection kit and applications thereof. The kit comprises (1) three pairs of amplification primers for the quantitative methylation of
The EpiQuik Quantitative PCR Fast Kit allows for a fast, specific, sensitive, and reproducible quantitative real time analysis of DNA samples. The novel hot start DNA polymerase contained in this kit reduces the overall time...
(iv) Bisulfite pyrosequencing (Pyroseq) applies sequencing by synthesis39 to single PCR amplicons obtained from bisulfite-converted DNA40,41. Second, relative DNA methylation assays measure DNA methylation by comparing samples to a suitable reference. This approach is mainly used for detecting ...
ResearchArea:DNAMethylation TargetApplication:PCRAmplification VesselFormat:96-WellPlate,Columns/Tubes 100%Guarantee:6months ProductOverview TheEpiQuik™QuantitativePCRFastKitallowsforafast,specific,sensitive,andreproducIBLequantitativerealtimeanalysisofDNAsamples. ThenovelhotstartDNApolymerasecontainedinthiskitreducesth...
DNA methylation was analyzed in Dr. Yongmei Liu’s laboratory at Duke University. Bisulfite conversion was performed on 500 ng of DNA using the Zymo EZ DNA Methylation Kit, following the manufacturer's instructions. The converted DNA was then processed using PCR conditions optimized for Illumina’...
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Temperature gradient PCR was utilized for optimizing annealing conditions. Amplicon size and yield of multiplex reactions were evaluated on a Bioanalyzer instrument using the DNA 1000 Kit (both Agilent Technologies, Santa Clara, CA, USA) and the results were used to adjust primer concentrations. ...
method for detection and quantification of nucleic acids (DNA,& RNA (cDNA) levels. It is based on detection and quantification of fluorescence emitted from a reporter molecule at real time. This detection occurs during the accumulation of the PCR ...
(bisulfite treated normal leukocyte DNA), and a methylated control (bisulfite converted in vitro methylated human DNA). The amplification was carried out in 96-well plates and the PCR reaction and cycling conditions were according to the kit manual. Subsequent sample preparation and pyrosequencing ...
PCR round that is methylation status-specific. Within genes of the panel, the level of promoter hypermethylation as well as the incidence of promoter hypermethylation can be determined and the level of genes in the panel can be scored cumulatively. The QM-MSP method is adaptable for high ...