To gain insight into the accuracy of microbial measurements, it is important to evaluate sources of bias related to sample condition, preservative method and bioinformatic analyses. There is increasing evidence that measurement of the total count and concentration of microbes in the gut, or 'absolute...
Quantifying bias introduced by sample collection in relative and absolute microbiome measurements (vol 42, pg 328, 2024) 来自 国家科技图书文献中心 喜欢 0 阅读量: 12 作者:DG Maghini,M Dvorak,A Dahlen,M Roos,B Doyle,S Kuersten,AS Bhatt ...
We used 80 bacterial mock communities comprised of prescribed proportions of cells from seven vaginally-relevant bacterial strains to assess the bias introduced in the sample processing pipeline. We created two additional sets of 80 mock communities by mixing prescribed quantities of DNA and PCR ...
One possible shortcoming of using a very detailed classification of ethnicities is that rare ethnicities will bias the class coverage times towards higher values (see Supplementary Fig. 8). In some extreme situations, a walker will take a considerably large amount of time to encounter a given ...
with respect to the pre-PCR pool distribution at 60x coverage. The blue distribution shows the effect of subsampling, while the further widening of the green curve with respect to the blue curve is attributed to stochastic bias in the PCR process. The sample size (number of unique sequences...
separately. Indeed,MIvalues stemming from different fits of the same allometric relationship(s) are not comparable to each other. A comparison would introduce biases comparable to those introduced by performing conditional predictions (see Methods for details). Whenever sample size allows it, we ...
ISIMIP3b refers to the future climate data of 5 CMIP6 GCMs bias-corrected and spatial-downscaled by Inter-Sectoral Impact Model Intercomparison Project phase 3b (Lange, 2019). 2.2 Data Site-level CH4 flux data were obtained from 35 chamber sites and 47 eddy covariance sites of various wetland...
Other biases also exist in the maps of soil moisture for the Tucson Basin that are associated with areas with low road density and bias in the direction of travel, such as in the southern part of the study region where there are few roads and where those roads that exist have are a ...
Secondly, this study relies on self-reported data, which may be subject to bias and inaccuracies. Future studies could use more objective measures to collect data, such as social media activity or mobile phone usage patterns. Thirdly, there was a low response rate for the survey during the ...
This bias is introduced from both the inability to capture tiny cell fragments discarded after centrifuging during the cell recovery process and the gating issues described. This may be especially important in the ablative HIFU groups; it is likely that the resulting mean of 23.4% viable ex vivo...