Quantification strategies in real-time PCR. In: A-Z of quantitative PCR. La Jolla, CA, USA: International University Line; 2003. Google Scholar Arezi B, Xing W, Sorge JA, Hogrefe HH. Amplification efficiency of thermostable DNA polymerases. Anal Biochem. 2003;321(2):226–35. Article CAS...
Overview of real-time RT-PCR strategies for quantification of gene rearrangements in the myeloid malignancies. Methods Mol Med 2006;125:27 - 68.Picard C, Silvy M, Gabert J. Overview of real-time RT-PCR strategies for quantification of gene rearrangements in the myeloid malignancies. Methods Mol...
Breeding for resistance is one of the best and ecofriendly strategies to manage the FHB. However, the existing methods used for screening genotypes, both under field and greenhouse conditions, often resulted in high experimental error, leading to inconsistent ranking of genotypes over years. In the...
Real-Time RT-PCR Quantification of Yellow Dwarf Virus Accumulation and Defense Gene ExpressionYellow dwarf virus (YDV)* is one of the principal diseases ... B Balaji,DB Bucholtz,HW Ohm,... - Barley Yellow Dwarf Disease: Recent Advances & Future Strategies ; An International Symposium Held at...
A Laboratory Challenge Method for Estimating Taura Syndrome Virus Resistance in Selected Lines of Pacific White Shrimp Litopenaeus vannamei Abstract Among the strategies being developed to improve survival and harvest yields in the farming of Pacific white shrimp Litopenneus vannumei is breedin... BL ...
Here, we describe sensitive qRT-PCR assays for quantification of early stage gametocytes, that complement existing late-stage assays, to enable understanding of epidemiological factors that driveP. falciparumtransmission success,and robust assessment of control strategies targeting sexual stages. The assays...
Lso is yet to be cultured and, therefore, effective control strategies depend heavily on the early detection of the pathogen via polymerase chain reaction (PCR) assays. In this study, two new assays for the detection of Lso in New Zealand field samples were developed, and compared with ...
NGS strategies have expanded our ability to investigate genomic phenomena by referencing mutations in cancer and diseases through large-scale genotyping, developing methods to map rare chromatin interactions (4C; 5C and Hi-C) and identifying chromatin features associated with regulatory elements (ChIP-...
Quantification of the effect of control strategies on classical swine fever epidemics Emergency vaccination during an epidemic of classical swine fever virus (CSFV) has become a serious option because of the ethical problems of strategies wi... D Klinkenberg,EVD Wind,EAM Graat,... - 《Mathematical...
Detection of cytomegalovirus DNA in human specimens by LightCycler PCR: melting point analysis is mandatory to detect virus strains with point mutations in... Human cytomegalovirus (CMV) has evolved numerous strategies for evading host immune defenses, including piracy of cellular cytokines. A viral ho...