However, until now the reconstitution ability of CB samples has been assessed by colony forming unit-culture (CFU-C) assay or by simply counting CD34 + cells. The present study aims at establishing a method capable of assessing the potential of T lymphopoieses of CB samples. Methods: CD34 ...
coli were initially selected based on colony morphology and then typed genetically by sequencing fragments of three diagnostic loci, fumC, gyrB and adk, used in the multilocus sequence typing (MLST) analysis of E. coli[48]. MLST proceeded by the colony PCR [49] using primer pairs: fumC ...
ISO 29981:2010 | IDF 220:2010 Milk Products—Enumeration of presumptive Bifidobacteria—Colony count technique at 37 degrees C https://www.iso.org/standard/45765.html Google Scholar ISO/IDF (International Organization for Standardization/International Dairy Federation), 2015 ...
The objective of this study was to develop an image-based cytometric methodology for the quantification of viable pathogenic yeasts, which can offer increased sensitivity and efficiency when compared to the traditional colony forming unit (CFU) assay. Live/dead yeast quantification by flow cytometry ha...
CFU, colony forming unit. EGFR, epidermal growth factor receptor. Full size image Effect of antibody removal and reprobing on CLIC signal We examined whether the CLIC assay is compatible with removal of antibodies followed by re-probing of the same cells. We measured EGFR by CLIC in HN4 and...
3D view Clip Gallery to capture publication quality images PDF analysis reports Export data tables to Excel Specification for CLIQS Operating systems: Windows XP, Windows Vista, Windows 7, 8 and 10.Processor: 1.4 GHzMemory: Minimum 1Gb, recommended 2GbFree hard disk space: Recommended 5GbFile for...
A single colony of strain S288C was inoculated into 10 ml of YPD (1%(w/v) yeast extract/2%(w/v) Bacto peptone/2%(w/v) glucose) and grown with shaking at 30°C for overnight. To prepare cells under a rich condition, they were resuspended in 100 ml of YPD at an OD600 of 0.1 ...
For colony numbers see Supplementary Table 1. Table 3 Plating scheme for counting of double transformants after selection 2 Full size table The percentage of double transformants was now calculated using eq.2. $$\frac{2\;\times\;No.\;colonies\;on\;SC\;-\;ura\;-\;leu}{No.\;colonies\...
Synaptic boutons are highly plastic structures undergoing experience-dependent changes in their number, volume, and shape. Their plasticity has been intensively studied in the insect mushroom bodies by manually counting the number of boutons in small reg
pyogenes strain samples and growth phase unspecific by harvesting them in both exponential and stationary phase. Four widely used bacterial counting techniques were used to accurately determine the number of bacteria in these samples; optical density at 620 nm, colony forming units (CFU), light ...