input.fastq.gz← The input long reads to be filtered (must be FASTQ format). | gzip > output.fastq.gz← Filtlong outputs the filtered reads to stdout. Pipe to gzip to keep the file size down. Filtlong has cut the original 1.3 Gbp of reads down to a much better 500 Mbp subset. Shor...
fragrans using the quality-filtered reads of Illumina data. The genome size was estimated based on the formula: Genome size = Modified k-mer number/average k-mer depth, where modified k-mer = Total k-mer number−error k-mer number and the average k-mer depth obtained from the...
To identify ERAS targets, we filtered for mRNAs that were upregulated in Ago2−/− cells and downregulated in wild-type (Ago2+/+) control cells for both ER stressors (Fig. 5a,b). BUSCA analysis of subcellular localization revealed that of the 187 candidates (Fig. 5c) 42% had GO ...
The sequenced reads were quality filtered (> Q30) by applying the FASTX tool kit. Data of each condition were subsequently mapped to the Hypom. monticulosa using tophat2 (Kim et al. 2013). Two mismatches were allowed. The resulting BAM-files were used for the BRAKER2 gene prediction ...
With the filtered data set, you use a Lookup transformation to determine whether the customer code already exists in the table. If the lookup is successful, data is updated in the destination table. If the lookup is not successful, an error branch is created for additional processing. In the...
Experiments on simulated and real NGS data have shown that reads with low sequencing quality could be identified and filtered. Possible contaminating sources could be identified and quantifiedde novo, accurately and quickly. Comparison between raw reads and processed reads also showed that subsequent ...
Using FastQC software and SOAPdenovo 2, the filtered data, respectively, were performed with quality control and sequence splicing. Following KEGG annotation... W Li,Y Han,X Yuan,... - 《Archives of Microbiology》 被引量: 1发表: 2017年 加载更多研究...
This assembly was realized through integration of a sequence-based physical map built using Whole Genome Profiling (WGP) with a whole genome sequence (WGS) assembly based on ~44X filtered PacBio reads of 20-kb fragment libraries. Assembly of the PacBio reads using HGAP (Chin , 2013) ...
421 total reads. These reads were quality filtered, and a total of 563,119 reads (ranging from 23,736 to 100,701 per sample) were selected as the most reliable reads for taxonomy assignment. On the whole, a total of 454,884 reads were classified to species level, ranging from 67.19 to...
Quality filtering of the raw reads was performed under specific filtering conditions to obtain high-quality clean tags according to fqtrim (v0.94). Chimeric sequences were filtered using Vsearch software (v2.3.4). After dereplication using DADA2, we obtained a feature table and sequence. Alpha ...