The desalted mixture was finally analyzed by direct infusion electrospray ionization (ESI) on a Thermo Fisher Scientific (Waltham, MA, USA) LTQ-Orbitrap Velos mass spec- trometer utilizing quartz emitters produced in house. A stainless steel wire was inserted through the back-end of the emitter...
We used in-house scripts to process a bed file containing all annotated alternative events which were obtained from VastDB’s UCSC Genome Browser Track Hub53. We extracted the splice site coordinates from exon skipping (HsaEX), alternative acceptor (HsaALTA), alternative donor (HsaALTD), intron...
sample Sample not damaged Inexpensive Limitations Expensive equipment Large amount of sample Crystallizable ligand/G4 required Sensitive to differences in conditions Expensive equipment Large amount of sample Additional methods needed for full characterization Interpretation is sometimes ambigu- ous induce the ...
The protein-containing fractions were incubated at 4 °C with 0.5 mg of TEV protease (produced in-house) per 1 L culture for 3 h at 25 °C to cleave the tag and then dialyzed overnight at 4 °C against 25 mM Tris-HCl [pH 8], 400 mM NaCl, 10 mM imidazole...
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Always a pre-production sample before mass production; Always final Inspection before shipment; 3.what can you buy from us? Wire,Cable 4. why should you buy from us not from other suppliers? Jinshui Wire & Cable Group is also a key company of high and new technology. We hav...
Single cell fluorescence intensity was unbiased quantified by Image J (version 1.54f) using in-house developed Macros, at least 200 cells were quantified for each group. And the quantification results were statistically analysed using GraphPad Prism 9.0. Immunofluorescence (IF) study of mitochondrial ...
Therefore, we introduce G4ShapePredictor (G4SP), an application featuring a collection of multi-classification machine learning models that are trained on a custom G4 dataset combining entries from existing literature and in-house circular dichroism experiments. G4ShapePredictor is designed to ...