C-q Z, Hu B, Zhu K-z, Zhang H, Leng Y-l, Tang S-z, Gu M-h, Liu Q-q: QTL mapping for rice RVA properties using high-throughput re-sequenced chromosome segment substitution lines. Rice Sci 2013, 20 (6) : 407-414. Publisher Full TextZhang C Q, Hu B, Zhu K Z, Zhang...
QTLMappingforRiceRVAPropertiesUsingHigh-Throughput Re-sequencedChromosomeSegmentSubstitutionLines ZHANGChang—quan,HuBing,ZHUKong—zhi,ZHANGHua,LENGYa—lin,TANGShu—zhu, GuMing—hong,L[uQiao—quan (KeyLaboratoryofPlantFunctionalGenomicsoftheMin&tryofEducation,JiangsuKeyLaboratoryofCropGeneticsandPhysiology ...
With the rapid advances of next-generation sequencing (NGS) technology, high-throughput sequencing-based marker discovery and genotyping have been quickly developed and adopted in many organisms5,6. The sequencing-based genotyping methods generally include multiplexing a proper number of samples with barc...
QTL analysis was conducted employing MapQTL V5.0 software using an interval mapping (IM) model. The significance thresholds were determined using 1,000 permutations. Calculation of the percentage of phenotype variance explained by each QTL (Expl. %) was achieved based on the population variance withi...
The mapping population consisted of 296 RILs derived from SN304 and YN15. Polymorphic markers were developed for genetic map construction using specific locus amplified fragment sequencing (SLAF-seq) technology. The genetic map included 18 groups and 3,053 loci spanning 1401.44 cM with an average ...
Library construction and high-throughput sequencing Genomic DNA was extracted from the two parents and the BC4F3population using a cetyl trimethylammonium bromide (CTAB) protocol [43]. Illumina HiSeq™ 2500 sequencing was used for DNA sequencing. The Hpy166II endonuclease was chosen to digest the...
rufipogon. J Crop Sci Biotech 13(4):205–212 Zhang C, Hu B, Zhu K, Zhang H, Leng Y, Tang S, Gu M, Lie Q (2013) QTL mapping for rice RVA properties using high-throughput re-sequenced chromosome segment substitution lines. Rice Sci 20(6):407–414 Submit your manuscript to a ...
Thus, a great deal of work is still needed to improve the genetic map density and QTL mapping accuracy by using high-throughput sequencing technologies and making full use of the reference Chinese cabbage genome. In addition, the currently availa- ble completed genomic sequences of Chinese ...
et al. SLAF-seq: an efficient method of large-scale de novo SNP discovery and genotyping using high-throughput sequencing. PLoS One 8, e58700 (2013). 25. Xu, X. et al. A high-density genetic map of cucumber derived from Specific Length Amplified Fragment sequencing (SLAF-seq). Front. ...
利用HighMap软件对RIL群体开发的高密度分子标签进行遗传图谱构建,构建了包含6,297个SLAF标记的高密度遗传图谱,总图距为2,945.26cM,平均遗传距离为0.47cM(见图2)。 [0068]实施例4 [0069]qSW20‑1初定位 [0070]利用QTL Icimapping V4.1软件结合齐黄34×东生16群体表型数据和分子数据,进行大豆百粒重的QTL定位(...