上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。 慢病毒滴度测定 qRT-PCR 品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量 Clontech 631235 Lenti-X qRT-PCR Titration Kit 200 Rxns ￥6,010 收藏产品 加入购物车 → → → → → → qRT-...
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。 microRNA定量(qRT-PCR) 品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量 Clontech 638315 Mir-X miRNA First-Strand Synthesis Kit 60 Rxns ￥4,820 ￥3,615 Clontech 638313 Mir-X miRNA First-...
These primers are compatible with all lentiviral vectors sold by Takara Bio and most commonly used lentiviral vectors. If you are unsure whether this kit is compatible with your vector, please send your vector sequence to Technical_Support@takarabio.com, and our support team will be happy to ...
microRNA是一类被广泛关注的非编码RNA,长度为22 nt左右,对植物和动物的基因表达有着非常重要的调控作用。TakaRa 宝生物提供用于microRNA定量检测的Mir-X™系列产品,性价比较高,能够满足大部分研究人员的实验需要。 产品特点 · 通用的加A尾反转录原理,一次反转样品中的不同microRNA ...
然后逆转录。常用的是Takara和罗氏逆转录试剂盒,在这里以比较实惠的Takara试剂盒为例。将1ugRNA根据如下体系(20ul)和程序逆转为cDNA。 取出cDNA后加入180ulddH2O于-20℃保存待用。逆转录是傻瓜式操作,按照说明书做就行,没有什么特别容易失误的地方,这里不啰嗦了。
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These primers are compatible with all MMLV vectors sold by Takara Bio (e.g., Q-series and L-series vectors) and most commonly used retroviral vectors. However, vectors carrying a significantly modified packaging signal, like the one carried by MSCV vectors, would be incompatible with the kit...
PrimeScript Reverse Transcriptase是Takara公司依据M-MLV (Moloney Murine Leukemia Virus)来源的RTase开发的一种反转录酶。Primescript的灵敏度和特异性有大量的文献支持。比如,该酶在total RNA pool中可以检测到10-21 mol miRNA,另外,该酶可以特异性检出含有单碱基错配的miRNA分化(Yao, B. et al. (2009) RNA 15...
TaKaRa Ex Taq HS (5 U/µl) PrimeScript RT Enzyme Mix II RNase Free dH2O ROX Reference Dye (50X conc.) ROX Reference Dye II (50X conc.) One-step qRT-PCR with TB Green detection. One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time); RR086A 2X One Step TB Green RT...
tm rt reagent kit with gdna eraser(perfect real time)购自takara公司):反应体系和反应程序见表2。 [0056] 表2 [0057][0058] 4.qrt-pcr检测 [0059] (1)引物设计 [0060] 根据ncbi中linc00707序列设计qrt-pcr引物,见表3。采用gapdh作为质内参,u6为核内参,进行qrt-pcr检测,每份样品重复3次。对总rna、胞...