Troubleshooting abnormal amplification plots Here are some examples of observed variations from the standard amplification curve, along with potential causes and corrective steps. Observation: exponential amplification in the no template control (NTC) Potential causes: Contamination from laboratory exposure to...
用于qPCR 的稳定型 Blood-to-CT™ 核酸制备试剂盒使得研究人员能够使用直接收集到 Tempus™ 血液 RNA 管中的 500 µl 人全血等份试液进行表达分析,无需进行传统 RNA 纯化。这一简单程序可产生能够直接进入逆转录和实时荧光定量 PCR 分析的裂解物,且其灵敏度和特异性与传统纯化 RNA 相当。该试剂盒可节省...
Important notes -This document is only a quick start guide, please refer to the official instrument user manual for in depth instructions. Contact us if you have questions or need help with troubleshooting.-Check the compatibility of your mastermix with this instrument. In particular, the passive...
Achieve high efficiency of probe-based qPCR under fast or standard cycling conditions Minimize troubleshooting─PrimeTime qPCR Probes are quality controlled via electrospray ionization mass spectrometry (ESI-MS)* and HPLC-purified * With the exception of mixed base oligos, which could potentially represent...
Related Topics: High Resolution Melting, Real-Time PCR Data Analysis, and Real-Time PCR Troubleshooting.Page Contents Step-by-Step Design Strategy for qPCR Assays When designing a qPCR assay, follow these steps: Check the literature and databases (such as www.rtprimerdb.org) for existing primer...
Troubleshooting What should I do if I have extra peaks in my melt curve? What does this mean? My amplification curves have a funny shape. What is causing this? What can I do to improve the sensitivity of my assay? How do I do a no-RT control r...
A Quick Guide for troubleshooting qPCR data SUN™ fluorophore Improve your assays with customizable oligos and probes containing cost-effective locked nucleic acids Genotyping: Terms to know Consider SNPs when designing PCR and qPCR assays A qPCR master mix that can be shipped at ambient temperatures...
[5] Protocol 1. Standard reaction set up 2. Cycling conditions 2-1. Real-time PCR conditions using Applied Biosystems 7900HT 2-2. Real-time PCR conditions using Roche LightCycler 1.1 [6] Related Protocol cDNA synthesis [7] Troubleshooting [8] Related products ...
Can I use shorter cycling times? What is the fluorescent, double-stranded DNA binding dye in the Luna® qPCR master mix? 问题解决指南 Luna® qPCR Troubleshooting Guide 发表在 neb酶 | 标签为 luna、neb、new、qpcr、预混液 文章导航 ← 早期文章搜索...
荧光定量PCR技术的国际标准化指南•IntroduceMIQE•QPCRworkflowandoptimization Page2 ApplicationsusingQPCR MatureapplicationsGeneExpressionMicroarraydatavalidationApplicationinNGS GMOdetection Veterinaryanalysis(viral/bacterialdetection)Genotyping&SNPsanalysisGeneexpressionknockdown(siRNA)PathogenDetection&RareRNAdetection...