To calculate this value manually, plot a graph with the log of each sample dilution against the averageCq value. Excel will calculate the R2value, which you can see by ensuring ‘Display equation on chart’ is selected in chart options. Standard Deviation for Cq Values Performing replicates hel...
Since many available tools do not provide a suitable graphical representation of the calculated results, Microsoft Excel is often used to create figures which require manual import and/or conversion of data. QPCR combines the calculation and presentation of results into one single tool which reduces ...
standard curve modelDraft method C is a standardized method for quantifying E. coli densities in recreational waters using quantitative polymerase chain reaction (qPCR). The method includes a Microsoft Excel workbook that automatically screens for poor-quality data using a set of previously proposed ...
(in cells F3, E129 or BE9 of the "UMES.xls" file) and proceeds to correct them by either altering/correcting user input values, ad- justing standard curve ranges, adjusting the "Sample Aim- ing Device" (in "UMES.xls" file cell range AX12:BK38) - all in conjunction with the ...
2)单击Standard Curve查看标准曲线的R2值、扩增效率Eff%,如图3-8所示。 图3-8 3)单击右上方View Well Table查看Ct值和浓度,如图3-9。 将数据导出到Excel表中,导出方法为:点击File,选择File菜单中的Export,在弹出的对话框中的ExportProperties中勾选Results,然后根据需要设置Export File Name和Export File Location...
2)单击 Standard Curve 查看标准曲线的R2值、扩增效率Eff%,如图3-8所示。 图3-8 3)单击右上方View Well Table查看Ct值和浓度,如图3-9。 将数据导出到Excel表中,导出方法为:点击File,选择File菜单中的Export,在弹出的对话框中的ExportProperties中勾选Results,然后根据需要设置Export和Export。设置完毕,点击Start ...
Starting the run -Wake-up the instrument from the touch screen (or switch on if needed). If the instrument was already on, make sure no run is still ongoing.-Open the “QuantStudio 6 and 7 Flex Real-Time PCR System software”.-“New Experiment” button “From template”.-Then, ...
Since the supplemental information from this research provided an excel template for calculating bias and precision, we can directly import the amplification curve data from two data sets analyzed by the CqMAN system into the excel template to obtain the calculated values of the two indicators. The...
(NFnand NFn+1) was calculated to define the optimal number of reference genes. When the V value was below the cut-off of 0.15, it was not necessary to include additional reference genes7. The NormFinder add-in for Microsoft Excel was used to estimate both intragroup and intergroup ...
1.2.3 候选内参基因的稳定性分析 使用 Microsoft Excel 2016 软件对实时荧光定量 PCR 产生的原始 Ct 值进行整理, 计算每个样本的平均 Ct 值. 使用 geNorm[22],NormFinder[23] 和 BestKeeper[24] 软 件对 8 个候选内参基因的稳定性进行分析.其中, geNorm 和 NormFinder 软件以 2- △ Ct 进行分析,△Ct= Ct...