Support Protocol 4: Instrument Settings Platinum qPCR SuperMix for SNP Genotyping can be used with real-time qPCR instruments that can detect three colors (one for each SNP, plus one for ROX Reference Dye). Supported real-time instruments include the ABI P...
The buffer is also optimized for increased resistance to reaction inhibitors originating from sample material or nucleic acid purification steps. Online resources thermofisher.com/lyo-ready For further information, contact MDxenzymes@thermofisher.com Protocol Please follow the instructions below to prepare ...
Furthermore, the protocol for gDNA removal with ezDNase takes as little as two minutes and does not require a dedicated enzyme inactivation step. Therefore, the workflow for cDNA synthesis involving gDNA removal with the SuperScript IV VILO Master Mix can be significantly shorter than with ...
pdf PrimeTime One-Step RT-qPCR Master Mix protocol (391 KB) Brochures and flyers pdf PrimeTime One-Step RT-qPCR Master Mix Product Brochure (481 KB) Handbooks Real-Time PCR handbook Safety data sheets Search for MSDS by product name or part number Certificates of analysis (COAs) Find...
Additionally, while the ability to optimize both the RT and qPCR steps separately can improve sensitivity and efficiency, it comes with the drawback of having to optimize two reactions instead of one. Lastly, the two-tube protocol cannot be as easily adapted to automated, high-throughput ...
When you want rapid and cost-effective results in a single-tube protocol, utilize our One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time). This kit is available in both 100 and 500 reaction sizes. This method detects fluorescence produced during the amplification process by adding...
PCR反应的扩增效率严重的影响到PCR的结果,同样在qRT-PCR中,扩增效率对定量的结果尤为重要,除去反应液(reaction buffer)中其他物质及机器和protocol带来的影响,引物的质量对于qRT-PCR的扩增效率影响也非常大,为了保证结果的准确性,无论是相对荧光定量还是绝对荧光定量均需要对引物的扩增效率进行检测,而公认的有效的qRT-...
Anyone using the procedures in this protocol does so at their own risk. Cold Spring Harbor Laboratory makes no representations or warranties with respect to the material set forth in this protocol and has no liability in connection with the use of these materials. Materials used in this protocol...
before explaining qPCR data analysis, we need to quickly discuss how to determine reaction efficiency and specificity. You don't need to perform these steps after every qPCR experiment, but should always validate these two values when setting up a new qPCR protocol or changing your current workflo...
These steps are repeated for each PCR cycle. When using intercalating dyes, such as SYBR Green I (Thermo Fisher Scientific), primer-dimers and non-specific products will also contribute to fluorescence. In contrast, the 5′ nuclease assay is specific and fluorescence will only be produced for ...