Forensic Science International GeneticsKanthaswamy S, Premasuthan A, Ng J, Satkoski J, Goyal V. Quantitative real-time PCR (qPCR) assay for human-dog-cat species identification and nuclear DNA quantification. Forensic Sci Int Genet. 2012; 6: 290-295....
The real-time quantitative polymerase chain reaction (qPCR) is probably the most common molecular technique in use today, having become the method of choice for nucleic acid detection and quantification and underpinning applications ranging from basic research through biotechnology and forensic applications...
(HVR)I and II, which was aimed at increasing the discrimination power of the mitochondrial DNA (mtDNA) typing in forensic casework, and confirming ... D Huang,C Gui,S Yi,... - 《Journal of Huazhong University of Science and Technology (Medical Sciences)》 被引量: 19发表: 2010年 Molecul...
Forensic laboratories commonly have to analyze samples that contain minute amounts of DNA (Low Copy Number DNA). LCN samples can be amplified with increased numbers of PCR cycles, but stochastic variations of their profiles are generally observed. Here, we compare some characteristics of the AmplST...
Research groups have screened out some sets of miRNA markers that can be used in forensic body fluids identification up to now. Most of those miRNA markers could not be repeated in different labs. The markers confirmed by SYBRgreen always could not be confirmed by another group in Taqman. ...
Real-time PCR is used to detect specific DNA sequences. This method uses fluorescent dyes or probes to determine the amount of amplified material in your sample. Find a variety of master mixes, plus real-time PCR instruments,assays, and kits for quantifying your qPCR results. ...
In conclusion, the ARMS-qPCR test established in this study can quickly, simply and accurately detect the c.2T>C mutation of SLC25A13 gene, which is helpful for the diagnosis of citrin deficiency (CD). KEYWORDS: Amplification-refractory mutation system quantitative real-time polymerase chain ...
Reaction (qPCR) is a highly adaptable experimental technique used across biology and medicine to measure the amounts of nucleic acids (DNA or RNA). tidyqpcr is a software package for qPCR data analysis that builds on the tidyverse collection of data science tools in the R programming language....
StopwatchI will admit that over the years, I have watched various crime scene investigation shows and read several books by Kathy Reichs and Patricia Cornwell because I was fascinated by forensic science. These same books and shows are a guilty pleasure because as a scientist, I know these por...
Forensic Analysis Quantitation of DNA can play a primary role in forensic investigation and analysis. Biological samples may often be extracted from the crime scene, and profiling can be done in laboratories with the help of the right kits. The amplification of the samples as well as the use ...