计算2-DDCt值的公式如下：2-DDCt=2-[(Ct目的基因-Ct内参基因)处理组-[(Ct目的基因-Ct内参基因)对照组]。举例来说，如果Cdk5在处理前后样品中的Ct平均值分别为25和22.1，内参GAPDH在处理前后样品中的Ct平均值分别为18.2和18.3，则Cdk5表达的变化可以用2-DDCt公式计算得出，结果显示处理组Cdk5的...
sample_groups<-c('Calibrator',(sprintf('%s%s',c('A','B'),rep(c(0,8,16),each=2))) > sample_groups [1] "Calibrator" "A0" "B0" "A8" "B8" "A16" "B16" 即将样品划分为了以上7种,根据自己需要调节 get_ddCt_all<-function(rt_ct,sample_groups=sample_groups){ddCt_all<-data.fram...
#使用ddCt包分析基因表达差异 library("ddCt") showClass("InputFrame") coreData <- data.frame(matrix(nrow = nrow(datax), ncol = 4)) colnames(coreData) <- c("Sample", "Detector", "Ct", "Platename") ddct.raw <- new("InputFrame") ddct.raw@coreData <- coreData ddct.raw@coreData$...
22DDCTMethod KennethJ.Livak*andThomasD.Schmittgen AppliedBiosystems,FosterCity,California94404;and†DepartmentofPharmaceutical Sciences,CollegeofPharmacy WashingtonStateUniversity,Pullman,Washington99164-6534 摘要: 现在最常用的两种分析实时定量PCR实验数据的方法是绝对定量和相对定量。绝对定量通过标准曲线计 ...
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 22DDCT Method Kenneth J. Livak* and Thomas D. Schmittgen Applied Biosystems, Foster City, California 94404; and †Department of Pharmaceutical Sciences, College of Pharmacy Washington State University, Pullman, ...
ddCt: the ddCt algorithm for the analysis of quantitative real-time PCR (qRT-PCR). (2021). R package version 1.48.0. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods. 2001;25(4):402–8. https://...
get_ddCt(get(paste('delta_Ct_',sample_groups[-1][i],sep="")))#循环调用变量}#得到ΔΔCtfor(iin1:length(sample_groups[-1])){ddCt_all<-rbind(ddCt_all,get(paste('ddCt_',sample_groups[-1][i],sep="")))}#合并所有ΔΔCtddCt_all<-ddCt_all|>mutate(quantity=2^(-(ddCt_all...