根据MIQE(Minimum Information for Publication of Quantitative Real-Time PCR Experiments)的指导方针,qPCR为Quantitative real-time PCR的简称,RT-qPCR指的是Reverse transcription–qPCR,RT-PCR仅用来表示Reverse transcription polymerase chain reaction,而不是Real-time PCR,但是有少数作者并没有坚持这一原则。