实验方法:DNA定点突变技术(Q5 Site-directed Mutagenesis) 是指通过聚合酶链式反应(PCR)等方法向目的DNA片段(可以是基因组,也可以是质粒)中引入所需变化(通常是表征有利方向的变化),包括碱基的添加、删除、点突变等。定点突变能迅速、高效的提高DNA所表达的目的蛋白的性状及表征,是基因研究工作中一种非常有用的手段...
Why do I not see my PCR product after using the Q5® Site-Directed Mutagenesis Kit? What plasmid sizes can be amplified using the Q5® Site-Directed Mutagenesis Kit? Do I need to purify my plasmid before or after the KLD reaction when using the Q5® Site-Directed Mutagenesis Kit? Ho...
Q5 Site-Directed Mutagenesis Kit(Without Competent Cells)产品规格:10T发货周期:已停产产品价格:已停产Q5定点突变试剂盒可以在2小时内快速对双链质粒DNA进行定点突变,该试剂盒使用稳定的Q5热启动超保真DNA聚合酶,结合客户自行设计的引物,在各种质粒中引入替换、缺失和插入突变。为保证效率,将产物转入试剂盒提供的高效...
英文名: Q5 Site-Directed Mutagenesis Kit (without competent cells) 英文别名: 中文名: Q5 定点突变试剂盒 (不含感受态细胞) 中文别名: Q5 定点突变试剂盒 (不含感受态细胞) CBNumber: CB95817855 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商2 ...
Q5 Site-Directed Mutagenesis 我的实验目的1 :是给VDR的N端加150个碱基,因为此试剂盒只能一次最多加100个碱基,所以我们分2次试验, F1:gtcgatggtgctcagaactgctggagtggaggggATGGAGGCAATGGCGGCC R1:agccaatcgctccttttcttattcctccactccatTATCGATGAATTCGCGGCCG...
Learn how to create substitutions, deletions or insertions in 3 easy steps with the Q5 Site-Directed Mutagenesis Kit. Script The Q5 Site-Directed Mutagenesis Kit allows rapid site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Sta...
Site Directed Mutagenesis, Site Directed Mutagenesis, High-throughput cloning and automation solutions, Hot Start PCR, Long Range PCR, Fast PCR, High-Fidelity PCR, Multiplex PCR, Specialty PCR, Routine PCR, DNA Amplification, PCR & qPCR,
The Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid,site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours(Figure 1). The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerasealong with custom mutagenic primers to create insertions, delet...
Q5 SITE‐DIRECTED MUTAGENESIS KIT(WITHOUT COMPETENT CELLS) 中文名: 中文别名: CBNumber: CB64690997 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商4 Q5 SITE‐DIRECTED MUTAGENESIS KIT(WITHOUT COMPETENT CELLS)化学性质 安全信息 ...
Q5 Site-Directed Mutagenesis Kit2020/2/3 12:22:43 浏览:【品牌】: NEB 【产品货号】: E0554S 【价格】: 2159 元 【规格】: 10 rxns 【货期】: 请咨询产品介绍 产品说明 【品牌】: NEB 【产品货号】: E0554S 【价格】: 2159 元 【规格】: 10 rxns 【货期】: 请咨询 【储存温度】: $article_...