11.Amplification of longproducts:Whenamplifying products > 6 kb, it is often helpful to increase the extension time to 40–50 seconds/kb. 12.PCRproduct:ThePCR products generated using Q5 High-Fidelity DNA Polymerase have blunt ends. If cloning is the next step, then blunt-end cloning is rec...
11. Amplification of long products:When amplifying products > 6 kb, it is often helpful to increase the extension time to 4050 seconds/kb. 12. PCR product:The PCR products generated using Q5 High-Fidelity DNA Polymerase have blunt ends. If cloning is the next step, then blunt-end cloning ...
11.Amplification of longproducts:Whenamplifying products > 6 kb, it is often helpful to increase the extension time to 40–50 seconds/kb. 12.PCRproduct:ThePCR products generated using Q5 High-Fidelity DNA Polymerase have blunt ends. If cloning is the next step, then blunt-end cloning is rec...
|il Reaction 5XQ5 Buffer 5uliom1;; 10mhldhJTPs 0J5pl 1pl200pM 10|JMPrimer 2.550,5啊 10IJMReversePrrmpr 2.5S0.5pM T&mp : ateDNAvariableVariable<1,000rif Q5High-FidelityONAPolymeraseD^5ul05Hl002U仙 5X06HighGCEnhancerOptional) (WD (10Ml)g Njclease-FrecWalerto25plto50m Notes:Gently...
11. Amplification of long products:When amplifying products > 6 kb, it is often helpful to increase the extension time to 40–50 seconds/kb. 12. PCR product:The PCR products generated using Q5 Hig 17、h-Fidelity DNA Polymerase have blunt ends. If cloning is the next step, then blunt-...
Q5 High-Fidelity DNA Polymerase - Lab-JOT:Q5高保真DNA聚合酶实验室记Q5,实验,酶,DNA,高保真,high 文档格式: .pdf 文档大小: 7.15M 文档页数: 6页 顶/踩数: 0/0 收藏人数: 1 评论次数: 0 文档热度: 文档分类: 论文--毕业论文 文档标签:
Time Total 1 hr. 45 min. 5-10 min. 2 hrs. Time (6.7 kb plasmid) The use of a master mix, a unique multi-enzyme KLD enzyme mix, and a fast polymerase ensures that, for most plasmids, the mutagenesis reaction is complete in less than two hours. Figure 2: Q5 Site-Directed ...
Quantitative Polymerase Chain Reaction (PCR). QPF Quantitative Precipitation Forecast. NOT ``Qualitative.'' QPL Qualified Product Lists. Federalese. QPM Quasi-Phase Matching. Q-point Quiescent Point. QPSK Quadrature Phase-Shift Keying. QPSX Queue Packet and Synchronous circuit eXchang...
or complex templates 6 kb, it is often helpful to increase the extension time to 4050 seconds/kb.12. PCR product:The PCR products generated using Q5 High-Fidelity DNA Polymerase have blunt ends. If cloning is the next step, then blunt-end cloning is recommended. If T/A-cloning is ...
or complex templates 6 kb, it is often helpful to increase the extension time to 4050 seconds/kb.12. PCR product:The PCR products generated using Q5 High-Fidelity DNA Polymerase have blunt ends. If cloning is the next step, then blunt-end cloning is recommended. If T/A-cloning is ...