[Method] RT-PCR method was used to ... Y Zhang,YY Zheng,FY Zhao,... - 农业科学与技术(英文版) 被引量: 10发表: 2010年 SETUP OF A TEST RIG FOR THE CHARACTERIZATION OF DEVICES FOR ACOUSTIC MEASUREMENTS IN HOT FLOW The influence of different variables, e.g. temperature and positioning ...
Such facets are: a) Method of RNA or DNA isolation, purification and storage; b) DNase or RNase treatments and reverse transcription (RT) reaction formulations; c) Dilutions of all samples beyond the point where inhibition of RT and/or PCR is expected; d) Use of all samples, per qPCR ...
Quantitative real-time PCR (qRT-PCR; StepOnePlus™ Real-Time PCR System, Thermo Fisher Scientific, Waltham, MA, USA) was used to detect the expression pattern of genes related to photosynthesis and photorespiration, which were explored from transcriptome data of C. hongkongensis subsp. tonkinensis...
介绍了分子生物学前沿技术聚合酶链式反应(PCR)的原理、方法及应用。 The principle, method and application of Polymerase Chain Reaction (PCR) was introduced in this paper. 20. 22kb 为使链式反应刚好能够维持下去,反应堆要有一个最起码的尺寸。(目的状语) There is a minimum size for the reactor at ...
该文就半定量RT-PCR法的检测步骤及技术的关键因素(总RNA提取、引物设计、循环数的确定和内参的选择等)进行综述。 15. 44kb This paper introduced the source of exhaust gas from cathodic electrodeposition primer in baking, and gave the design principle of disposal equipments. 介绍了阴极电泳漆烘干过程中...
Quantitative reverse transcription PCR (RT-PCR) To correlate the DNA copy-number status of candidate genes with their mRNA expression, 5 μg of total RNA from leukemia cells and a reference from normal T lymphocytes (pool of 5 individuals, age 16-28 years) were used as a template for rever...
Yes, please follow the Supplementary Protocols at the links below: Rotor-Gene SYBR Green PCR Kit: 'Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR Kit' (PCR106) Rotor-Gene SYBR Green RT-PCR Kit: 'Rotor-Gene software setup for the Rotor-Gene SYBR Green RT-PCR Kit' (PCR107...
In principle, the PRRT2-dependent decrease in Pr can be due to either an uncoupling between the Ca2+ sensor and the SNARE complex or a decreased Ca2+ influx or both. The observation that PRRT2-depleted neurons are totally insensitive to an increase in the extracellular Ca2+ concentration (...
The thermal shift assay was performed using the StepOne real-time PCR system (Applied Biosystems). For the thermal shift assay of the Q-body, the purified Fab was added to a final concentration of 70 nM in a 30 mL PBST containing 1% BSA. The change in fluorescence was monitored using ...
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