(Liu, Ramsey, et al., 2011). The consumption of H2O2by bacteria may be an important mechanism by which streptococci can tolerate theoxidative stressinduced by self-produced H2O2. For example,A. oriscatalase protectsS. gordoniifrom protein oxidation mediated by its own H2O2(Jakubovics, Gill, ...
113 AAO catalyzes the in situ oxidation of alanine back to pyruvate in the presence of molecular oxygen (Figure 29). As a result, the AAO approach eliminates the use of stoichiometric amounts of pyruvate for the regeneration of PLP. This serves to reduce the cost of the process (making ...
OxidationMechanismThe kinetics of oxidation of pyruvate by diperiodatoargentate( III) ion (DPA) has been studied spec-trophotometrically in alkaline medium. It was found that the reaction order with respect to both DPA and pyruvate is unity and the rate equation can be expressed asThe rate ...
2k. Most proteins were downregulated and involved in fatty acid synthesis and oxidation. Proteins within the pyruvate dehydrogenase complex were downregulated, while pyruvate kinase isozymes R/L, involved in synthesizing pyruvate, were upregulated. Together these data indicate a clear metabolic effect of...
3b,c). These results illustrate that the activity of the human HPD enzyme should preferentially be monitored at 37 °C and at the earliest of 17 hours after induction in order to allow the completion of the auto-oxidation and self-polymerisation process of HGA into the melanin-like o...
6a) without affecting HEt oxidation (Fig. 6b). In S8 cells the glutathione precursor N-acetylcysteine (NAC) prevented galactose-induced LS cell death, whereas the vitamin E-derivative Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was ineffective (Fig. 6c, d). These ...
High levels of NADH markedly weaken the catabolism of pyruvate in microbes by inhibiting PDHc activity, while enhanced NADH oxidation to NAD+ increases the glycolysis flux [66]. Introducing an NADH or NAD+ regeneration system is a direct way to influence the NADH/NAD+ ratio. Intracellular NAD+...
This was indicative of the oxidation of ThDP to thiamin thiazolone diphosphate (TZD). Consequently TZD was modeled into the active site during subsequent rounds of refinement. This oxidation of ThDP to TZD has been observed previously in BFDC, as well as other ThDP-dependent enzymes, and has ...
indirect antioxidant and anti-inflammatory effects and regulation of classical intracellular signal transduction, it has been demonstrated that some polyphenols exert their therapeutic effects through emerging new mechanisms (e.g., reduction of de novo lipogenesis and increased fatty acid β-oxidation) [...