The tripeptide was quantitated using a competitive radioimmunoassay in which 125I-labeled thyrotropin-releasing hormone (TRH), is displaced from antibody specific to TRH (pyroGlu-His-Pro amide). The pyroGlu-Tyr-Pro amide was purified by passing the filtered extract through QAE-Sephadex A25 at ...
Antibodies from mice immunized with Abeta1-40/42 recognized Abeta1-40, 1-7 and 1-15 peptides; splenocytes proliferated upon restimulation with Abeta1-40/42 but not the Abeta pE3 peptides or KLH. IgG1 was the dominant isotype for both vaccines. Hippocampal fibrillar plaques were ...