一.质粒连接:将目的片段 P53,MAD2 导入酶切位点,构建新的质粒。 1. 对原质粒PX330-A,PX330-S,做一个Bbs I 酶切,电泳切胶回收,获得线性质粒DNA。(酶 切体系详见切胶回收Protocol) 质粒酶切体系:质粒 7ul ;Bbs I 酶 1ul; Buffer 10ul;ddH2O 32ul 配好后 37℃水浴 15min。 测浓度:Px330-A:10...
The Transmission Control Protocol (TCP) is designed to give optimal performance over links with low bit error rates (BERs) where loss of packets is caused primarily by congestion. TCP performs poorly on lossy links where the main cause of packet loss is corruption. In such situations, TCP is...
This article is devoted to the analysis of the half-duplex protocol from the point of view of its applicability in the meteor radio channel of information transmission. The effectiveness of meteor radio communication systems is determine... M Ekaterina,Z Sergey,T Kudryashova,... 被引量: 0发表...