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A-modified Eagle's medium (aMEM) (Bio-Whittaker, Belgium) containing 10–15% (v/v) fetal bovine serum (FBS, Gibco™, Thermo Fisher Scientific), 100–150 U/mL penicillin, and 0.1–0.2 mg/mL streptomycin (HyClone, UK), in a humidified atmosphere at 30–40 ◦C and 5–7% CO2 [4...