We concluded that the F193A and F193I mutations shifted the substrate specificity of P450cam by causing structural changes that were relayed from their surface position down to the vicinity of the heme. The altered substrate binding resulted in differential electron transfer kinetics between classes ...
Lectins with different binding specificities were used to determine if the NAs preferentially cleaved α2,3-linked or α2,6-linked sialic acids using fetuin as a substrate which carries both these linkages in a 2:1 ratio27. Specifically, Erythrina crista-galli (ECA), peanut agglutinin (PNA),...
Each mechanism of PM FA transport into cells produces a predictable pH change according to the physical and chemical properties of FA in membranes. The direction and speed of pH change depends on the mechanism by which FAs move across the bilayer. Using BCECF to monitor each change, we ...
The substrate 3-methylaspartate (wheat) was modeled into the amino acid binding pocket. B, schematic representation of A. C, superposition of SlgN1ΔAsub-AMPcPP with PheA, including the substrates of PheA (pale cyan). The amino acid binding pocket is closed by a loop of the Asub domain...
Regardless of the function behind pyrosome bioluminescence and the tissue localization, the exact mechanism has not been determined. Like other bioluminescent organisms, pyrosomes rely on a chemical reaction between a substrate (luciferin) and an enzyme (luciferase) to produce their light; however, ...
The third active site, commonly constituted by the Ser-Thr-[Ser- Gln-Thr] motif and involved in the scaffold of the N AD+ binding pocket formation [18], is replaced by Ser-Ala- Thr- motif in mART-JH002. Despite of the substitution of threonine by an alanine in this active site,...
Nevertheless, there was no substitution of the key amino acids in the substrate pocket and catalytic dyad site (Hara-Nishimura et al. 2005). The sequence similarity analysis in ten common wheat varieties ('Westonia', 'Lancer', 'CDC Landmark', 'Claire', 'Janz', 'Chinese Spring', '...
Enzymatic alkane hydroxylation reactions are useful for producing pharmaceutical and agricultural chemical intermediates from hydrocarbons. Several cytochrome P450 enzymes catalyze the regio- and stereo-specific hydroxylation of alkanes. We evaluated the substrate binding of a putative CYP alkane hydroxylase (...
The α1α2 loop terminates at the phosphopantethylated Ser36 and provides Phe28 which occupies a key position in the acyl binding site, whereas a loop comprising helix α3 forms a lid over one of the walls of the hydrophobic pocket. In our model, the activated part of the substrate is...
WP_009885703.1 MG040 PSE YES (b/w 32–33) TMH = 0 ABC transporter substrate-binding protein PnrA-like Domain IPR003760 Hypothetical Protein yes 0.761 WP_009885565.1 MG468 PSE YES (b/w 33–34) TMH = 8 NO ABC transporter permease yes 0.668 WP_009885925.1 ptsG MG069 PSE YES (b/w 36–...