aAn enzyme-linked immunosorbent assay (ELISA) using recombinant nucleoprotein (rNP) was reported for the detection of immunoglobulin G (IgG) antibodies to Crimean–Congo hemorrhagic fever (CCHF) virus (CCHFV). The immunoglobulin M (IgM)-capture ELISA was developed for the diagnosis of CCHFV infecti...
Reactions of the HA antigens are detected through serum of influenza A (H1N1) vaccinated people. An indirect enzyme-linked immunosorbent assay (Elisa) method is established for detecting the influenza A (H1N1) HA antibodies, and the antibody types are further identified. According to the invention...
What is an ELISA test, and what is the use of this test in biology? Describe in details all criteria for a northern blot? What is the process of mass spectrometry? Explain. Explain the principle of the CH50 assay and its uses Explore our homework questions and answers library ...
What would be an appropriate negative control sample for your Elisa assay? What is the above-normal amount of crystals in the urine? Why might a patient's urine volume be high? What are the three steps used traditionally to test hormo...
Thus, these challenges also increase the complexity of validating the performance of the assay for its intended use. This paper describes the challenges encountered with multiplex LBAs, discusses the underlying causes, and provides solutions to help overcome these challenges. Finally, we provide ...
What is the purpose of using a positive control for the assay? What is a positive and negative control in an experiment? In the experiments done with laccase, describe the purpose of a spectrophotometer. What would be an appropriate negative contro...
What is the purpose of heat fixing in a simple staining experiment? When is parfocality achieved in microscopy? Which objective should be in place when you start to work with the microscope? What is the purpose of using a positive control for the assay? What process do scientists use as ...
Kumiko I!JIMA, Hiroaki NOBU- HARA and Munehiro HIRAYAMA (Dept. Maternal Child Health, Univ. Tokyo, Tokyo) Cytogenetic assay systems based on the detection of sister chromatid exchanges (SCEs) are widely advocated as a sensitive screening method for assessing genotoxic potential. While many agents ...
Precision Profiles of Calibration Curves The Precision Profile is a plot of the coefficient of variation of the calibrated concentration vs. the true concentration in log scale. The standard error of the calibrated concentration should include the variability of both the assay response and the calibrat...
To apply serology it was necessary to check the specificity of extracts from the salivary glands (SGE) from O. erraticus. For this, indirect ELISA, competitive ELISA and Western blot were used to assay the SGE from O. erraticus and their corresponding antisera against the SGE and respective ...