0.5 gm of Dextran T70 (Pharmacia) is dissolved in 1 liter of water and then 5 gm of activated charcoal (Norit A, alkaline; Fisher Scientific) is added. The suspension is stirred for 10 minutes at room temperature and then stored at 4° C. until use. To measure R:L complex, 4 parts...
Establishing clinically relevant single-cell (SC) transcriptomic workflows from cryopreserved tissue is essential to move this emerging immune monitoring technology from the bench to the bedside. Improper sample preparation leads to detrimental cascades,
By the use of mouse monoclonal antibodies anti-TAC and 7G7.B6, which recognize different epitopes on the human IL-2R x chain, visualized by fluorescence microscopy using FITC-goat-anti-mouce Ig, we can determine whether the enzyme treatment leads to any change in the surface expression of ...
Isotype: Mouse IgG1, κ Immunogen: Rat GM130 aa. 869-982 Application: Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended) Target Molecular Weight: 130 kDa Concentration: 250 µg/ml RRID: AB_398142 Stora...
As was shown in a Table 2, TIF stimulates the growth of normal cells. Therefore, the possibility that TIF may be mitogenic and able to stimulate DNA synthesis in quiescent cells, such as serum-starved mouse cells, by interacting with the EGF receptor was investigated. It was found that both...