This protocol describes the purification of recombinant proteins fused to glutathione S-transferase (GST, GST-tagged proteins) by Glutathione Affinity purification. The GST tag frequently increases the solubility of the fused protein of interest and thus enables its purification and subsequent functional ...
Glutathione-Superflow Resin allows rapid affinity purification of GST-tagged proteins for batch, gravity flow, and FPLC applications. The resin is based on 6% cross-linked agarose, with glutathione covalently bound to the resin. It can withstand flow rates as high as 15 ml/cm2/min and medium ...
The scale of purification of GST-tagged proteins is dependent on the amount of protein in the preparation. A column size and total binding capacity should be chosen to approximately match the amount of protein to be purified. Very few non-tagged proteins are usually retained on the resin if ...
Glutathione S-transferase (GST) is a multifunctional phase II metabolic enzyme and a macromolecular protein with a molecular weight of 26kDa.
Erica modified the affinity chromatography template inAKTA start to reliably obtain high quality GST-tagged protein, suitable for use in her research work. Frac30 fractioncollector facilitated the collection of fixed volume fractions without any manual intervention. The affinity templatecan be used for ...
Because GST folds rapidly into a stable and highly soluble protein upon translation, inclusion of the GST tag often promotes greater expression and solubility of recombinant proteins than expression without the tag. In addition...
Monitoring the Expression, Purification, and Processing of GST-Tagged Proteins Using the Experion™ Automated Electrophoresis System... BR Laboratories,Hercules,X He,... 被引量: 0发表: 0年 Expression of a recombinant form of the V antigen of Yersinia pestis, using three different expression syst...
英文: Study on Method of Purification of GST-tagged Proteins from Inclusion Bodies 中文: GST融合蛋白包涵体纯化方法的探索 更详细... 英文: A New Method of Purification of Hyaluronic Acid from Bovine Vitreous Body 中文: 从牛眼玻璃体中分离纯化透明质酸的新方法 更详细... 英文: An improved metho...
We offer a variety of Ni-NTA, cobalt, glutathione, and other resins for purification of recombinant fusion proteins (His-tagged, GST-tagged) from cultures such as E. coli or Picchia, including multiple formats to accommodate screening, batch, pilot, and
purify GST-tagged proteins. The glutathione resin selectively binds the GST-tagged protein under normal conditions, allowing the specific protein of interest to be separated from whole cell extracts rapidly and efficiently. A high degree of purification can be achieved in just one chromatographic step...