hTF may have the protein sequence in NCBI Reference Sequence: NP_001984 (isoform 1) or NCBI Reference Sequence: NP_001171567.1 (isoform 2). Human tissue factor is also known in the art as thromboplastin, coagulation factor III, or CD142. The term “hTF” encompasses “full-length” ...
To analyze e-antigen, seventy-two hours after transfection, cells were harvested and lysed in a buffer (1% NP-40, 50 mM Tris-HCl and 1 mM EDTA) at 37° C. for 10 minutes. Activity of β-galactosidase in cell lysate was determined to normalize the variation of transfection efficiency amo...